RRC ID 51270
Author Yang CH, Yeh YJ, Wang JY, Liu YW, Chen YL, Cheng HW, Cheng CM, Chuang YJ, Yuh CH, Chen YR.
Title NEAP/DUSP26 suppresses receptor tyrosine kinases and regulates neuronal development in zebrafish.
Journal Sci Rep
Abstract Expression of neuroendocrine-associated phosphatase (NEAP, also named as dual specificity phosphatase 26, [DUSP26]) is restricted to neuroendocrine tissues. We found that NEAP, but not its phosphatase-defective mutant, suppressed nerve growth factor (NGF) receptor TrkA and fibroblast growth factor receptor 1 (FGFR1) activation in PC12 cells upon NGF stimulation. Conversely, suppressing NEAP expression by RNA interference enhanced TrkA and FGFR1 phosphorylation. NEAP was capable of de-phosphorylating TrkA and FGFR1 directly in vitro. NEAP-orthologous gene existed in zebrafish. Morpholino (MO) suppression of NEAP in zebrafish resulted in hyper-phosphorylation of TrkA and FGFR1 as well as abnormal body postures and small eyes. Differentiation of retina in zebrafishes with NEAP MO treatment was severely defective, so were cranial motor neurons. Taken together, our data indicated that NEAP/DUSP26 have a critical role in regulating TrkA and FGFR1 signaling as well as proper development of retina and neuronal system in zebrafish.
Volume 7(1)
Pages 5241
Published 2017-7-12
DOI 10.1038/s41598-017-05584-7
PII 10.1038/s41598-017-05584-7
PMID 28701747
PMC PMC5507855
MeSH Animals Cell Differentiation Dual-Specificity Phosphatases / genetics Dual-Specificity Phosphatases / physiology* Embryo, Nonmammalian / cytology* Embryo, Nonmammalian / metabolism Mitogen-Activated Protein Kinase Phosphatases / genetics Mitogen-Activated Protein Kinase Phosphatases / physiology* Morpholinos / pharmacology Motor Neuron Disease / genetics Motor Neuron Disease / metabolism Motor Neuron Disease / pathology* PC12 Cells Phosphorylation Rats Receptor, Fibroblast Growth Factor, Type 1 / antagonists & inhibitors* Receptor, Fibroblast Growth Factor, Type 1 / genetics Receptor, Fibroblast Growth Factor, Type 1 / metabolism Receptor, trkA / antagonists & inhibitors* Receptor, trkA / genetics Receptor, trkA / metabolism Retinal Diseases / genetics Retinal Diseases / metabolism Retinal Diseases / pathology* Signal Transduction Zebrafish / embryology* Zebrafish / genetics Zebrafish / metabolism Zebrafish Proteins / genetics Zebrafish Proteins / physiology*
IF 3.998
Times Cited 2
Zebrafish Tg(CM-isl1:GFP)