RRC ID |
51270
|
著者 |
Yang CH, Yeh YJ, Wang JY, Liu YW, Chen YL, Cheng HW, Cheng CM, Chuang YJ, Yuh CH, Chen YR.
|
タイトル |
NEAP/DUSP26 suppresses receptor tyrosine kinases and regulates neuronal development in zebrafish.
|
ジャーナル |
Sci Rep
|
Abstract |
Expression of neuroendocrine-associated phosphatase (NEAP, also named as dual specificity phosphatase 26, [DUSP26]) is restricted to neuroendocrine tissues. We found that NEAP, but not its phosphatase-defective mutant, suppressed nerve growth factor (NGF) receptor TrkA and fibroblast growth factor receptor 1 (FGFR1) activation in PC12 cells upon NGF stimulation. Conversely, suppressing NEAP expression by RNA interference enhanced TrkA and FGFR1 phosphorylation. NEAP was capable of de-phosphorylating TrkA and FGFR1 directly in vitro. NEAP-orthologous gene existed in zebrafish. Morpholino (MO) suppression of NEAP in zebrafish resulted in hyper-phosphorylation of TrkA and FGFR1 as well as abnormal body postures and small eyes. Differentiation of retina in zebrafishes with NEAP MO treatment was severely defective, so were cranial motor neurons. Taken together, our data indicated that NEAP/DUSP26 have a critical role in regulating TrkA and FGFR1 signaling as well as proper development of retina and neuronal system in zebrafish.
|
巻・号 |
7(1)
|
ページ |
5241
|
公開日 |
2017-7-12
|
DOI |
10.1038/s41598-017-05584-7
|
PII |
10.1038/s41598-017-05584-7
|
PMID |
28701747
|
PMC |
PMC5507855
|
MeSH |
Animals
Cell Differentiation
Dual-Specificity Phosphatases / genetics
Dual-Specificity Phosphatases / physiology*
Embryo, Nonmammalian / cytology*
Embryo, Nonmammalian / metabolism
Mitogen-Activated Protein Kinase Phosphatases / genetics
Mitogen-Activated Protein Kinase Phosphatases / physiology*
Morpholinos / pharmacology
Motor Neuron Disease / genetics
Motor Neuron Disease / metabolism
Motor Neuron Disease / pathology*
PC12 Cells
Phosphorylation
Rats
Receptor, Fibroblast Growth Factor, Type 1 / antagonists & inhibitors*
Receptor, Fibroblast Growth Factor, Type 1 / genetics
Receptor, Fibroblast Growth Factor, Type 1 / metabolism
Receptor, trkA / antagonists & inhibitors*
Receptor, trkA / genetics
Receptor, trkA / metabolism
Retinal Diseases / genetics
Retinal Diseases / metabolism
Retinal Diseases / pathology*
Signal Transduction
Zebrafish / embryology*
Zebrafish / genetics
Zebrafish / metabolism
Zebrafish Proteins / genetics
Zebrafish Proteins / physiology*
|
IF |
3.998
|
引用数 |
2
|
リソース情報 |
ゼブラフィッシュ |
Tg(CM-isl1:GFP) |