RRC ID 514
著者 Ote M, Mita K, Kawasaki H, Seki M, Nohata J, Kobayashi M, Shimada T.
タイトル Microarray analysis of gene expression profiles in wing discs of Bombyx mori during pupal ecdysis.
ジャーナル Insect Biochem Mol Biol
Abstract Wing discs of holometabolous insects undergo dramatic morphological changes during metamorphosis, a process that is controlled by the actions of hundreds of gene products. Using cDNA microarrays constructed from 5086 ESTs, we monitored the gene expression profiles in wing discs of Bombyx mori at 13 time points during pupal ecdysis (day-4 fifth instar larvae to day-0 pupae). Of the 5086 ESTs on the microarrays, 2998 ESTs had significant signals in more than half of the experiments. Of the 2998 ESTs, genes represented by 683 ESTs showed significant perturbations during pupal ecdysis. Genes previously known to be induced during metamorphosis were identified, including E75, Urbain, Chitinases, and cuticle proteins. The expressions of genes represented by 59 ESTs induced at the beginning of wandering contained genes predicted to be involved in protein degradation, amino acid metabolism, and amino acid transport. The expressions of genes represented by 147 ESTs induced after the ecdysteroid peak had a role in cuticle synthesis, pigmentation, ion transport, protein transport, and transcription regulation. The expressions of genes represented by 85 ESTs repressed after the ecdysteroid peak were predicted to be involved in nucleotide and nucleic acid metabolism and cell cycle. This indicates the involvement of several biological processes in wing disc development during metamorphosis.
巻・号 34(8)
ページ 775-84
公開日 2004-8-1
DOI 10.1016/j.ibmb.2004.04.002
PII S096517480400061X
PMID 15262282
MeSH Animals Bombyx / genetics* Bombyx / growth & development* Gene Expression Profiling* Gene Expression Regulation, Developmental Molting / genetics* Oligonucleotide Array Sequence Analysis* Pupa / genetics* Pupa / growth & development Wings, Animal / growth & development* Wings, Animal / metabolism
IF 3.827
引用数 26
WOS 分野 ENTOMOLOGY BIOCHEMISTRY & MOLECULAR BIOLOGY
リソース情報
カイコ EST database