論文 - 詳細
| RRC ID | 51484 |
|---|---|
| 著者 | Prior H, Jawad AK, MacConnachie L, Beg AA. |
| タイトル | Highly Efficient, Rapid and Co-CRISPR-Independent Genome Editing in Caenorhabditis elegans. |
| ジャーナル | G3 (Bethesda) |
| Abstract |
We describe a rapid and highly efficient method to generate point mutations in Caenorhabditis elegans using direct injection of CRISPR-Cas9 ribonucleoproteins. This versatile method does not require sensitized genetic backgrounds or co-CRISPR selection-based methods, and represents a single strategy that can be used for creating genomic point mutations, regardless of location. As proof of principle, we show that knock-in mutants more faithfully report variant-associated phenotypes as compared to transgenic overexpression. Data for nine knock-in mutants across five genes are presented that demonstrate high editing efficiencies (60%), a reduced screening workload (24 F1 progeny), and a rapid timescale (4-5 d). This optimized method simplifies genome engineering and is readily adaptable to other model systems. |
| 巻・号 | 7(11) |
| ページ | 3693-3698 |
| 公開日 | 2017-11-6 |
| DOI | 10.1534/g3.117.300216 |
| PII | g3.117.300216 |
| PMID | 28893845 |
| PMC | PMC5677160 |
| MeSH | Animals CRISPR-Cas Systems* Caenorhabditis elegans / genetics* Gene Editing / methods* Gene Knockout Techniques / methods* Genome, Helminth Point Mutation |
| IF | 2.781 |
| 引用数 | 12 |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 最多言及媒体 | X(Twitter) |
| 各媒体での言及数の合計 | 28 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 3.0 |
| リソース情報 | |
| 線虫 | tm1444 |