論文 - 詳細
| RRC ID | 5151 |
|---|---|
| 著者 | Tsuchida Y, Kimura S, Suzuki N, Inui M, Yukawa H. |
| タイトル | Characterization of a new 2.4-kb plasmid of Corynebacterium casei and development of stable corynebacterial cloning vector. |
| ジャーナル | Appl Microbiol Biotechnol |
| Abstract |
A new plasmid pCASE1 was isolated from Gram-positive Corynebacterium casei JCM 12072. It comprised a 2.4-kb nucleotide sequence with three ORFs, two of which were indispensable for autonomous replication in Corynebacterium glutamicum. Homology search identified these two ORFs as repA and repB, areas coding proteins involved in plasmid replication. repA sequence showed high similarity to theta-replicating Escherichia coli ColE2-P9 plasmids and even higher similarity to plasmids derived from Gram-positive bacteria belonging to a subfamily of this ColE2-P9 group. An E. coli-C. glutamicum shuttle vector was constructed with pCASE1 fragment including repA and repB to transform C. glutamicum and showed compatibility with corynebacterial plasmids from different plasmid families. The copy number of the shuttle vector in C. glutamicum was 13 and the vector showed stability for 102 generations with no selective pressure. |
| 巻・号 | 81(6) |
| ページ | 1107-15 |
| 公開日 | 2009-1-1 |
| DOI | 10.1007/s00253-008-1746-1 |
| PMID | 18936936 |
| MeSH | Corynebacterium / genetics* Corynebacterium glutamicum / genetics DNA Replication DNA, Bacterial / genetics Escherichia coli / genetics Genes, Bacterial Genetic Vectors* Genetics, Microbial / methods* Molecular Biology / methods* Open Reading Frames Plasmids* Sequence Analysis, DNA Sequence Homology, Amino Acid |
| IF | 3.53 |
| 引用数 | 15 |
| WOS 分野 | BIOTECHNOLOGY & APPLIED MICROBIOLOGY |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 最多言及媒体 | Patent(IFI CLAIMS) |
| 各媒体での言及数の合計 | 3 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| 一般微生物 | JCM12072 |