| RRC ID |
51528
|
| Author |
Sood P, Murthy K, Kumar V, Nonet ML, Menon GI, Koushika SP.
|
| Title |
Cargo crowding at actin-rich regions along axons causes local traffic jams.
|
| Journal |
Traffic
|
| Abstract |
Steady axonal cargo flow is central to the functioning of healthy neurons. However, a substantial fraction of cargo in axons remains stationary up to several minutes. We examine the transport of precursors of synaptic vesicles (pre-SVs), endosomes and mitochondria in Caenorhabditis elegans touch receptor neurons, showing that stationary cargo are predominantly present at actin-rich regions along the neuronal process. Stationary vesicles at actin-rich regions increase the propensity of moving vesicles to stall at the same location, resulting in traffic jams arising from physical crowding. Such local traffic jams at actin-rich regions are likely to be a general feature of axonal transport since they also occur in Drosophila neurons. Repeated touch stimulation of C. elegans reduces the density of stationary pre-SVs, indicating that these traffic jams can act as both sources and sinks of vesicles. This suggests that vesicles trapped in actin-rich regions are functional reservoirs that may contribute to maintaining robust cargo flow in the neuron. A video abstract of this article can be found at: Video S1; Video S2.
|
| Volume |
19(3)
|
| Pages |
166-181
|
| Published |
2018-3-1
|
| DOI |
10.1111/tra.12544
|
| PMID |
29178177
|
| MeSH |
Actins / metabolism
Animals
Axonal Transport*
Caenorhabditis elegans
Drosophila
Endosomes / metabolism
Mitochondria / metabolism
Synaptic Vesicles / metabolism
|
| IF |
4.045
|
| Times Cited |
8
|
| Resource |
| C.elegans |
tm1552 |
