RRC ID 51583
Author Ernst J, Grabiec U, Greither T, Fischer B, Dehghani F.
Title The endocannabinoid system in the human granulosa cell line KGN.
Journal Mol. Cell. Endocrinol.
Abstract Ovarian steroidogenesis is embedded in a sensitive network of regulatory mechanisms crucial for human fertility. The endocannabinoid system (ECS) represents an intrinsic modulating system involved in the regulation of endocrine functions. In the present study we characterized the ECS in the human granulosa cell line KGN and its impact on gonadotropin sensitivity and steroid hormone synthesis under basal and FSH-stimulated conditions. Expression studies were performed and estradiol was measured. CB1, CB2, DAGL, FAAH, GPR55, MAGL, NAPE-PLD and TRPV1 were expressed without FSH-dependent effects. Treatment with selective cannabinoid receptor agonists reduced basal but not FSH-stimulated estradiol and CYP19. Progesterone was not altered by ECS manipulation. CB1 agonist changed the expression of miRNAs associated with granulosa cell function, e.g. miR-23a, miR-24, miR-181a and miR-320a. Present data indicate a modulating role of the intrinsic ovarian ECS in the regulation of estradiol synthesis.
Volume 423
Pages 67-76
Published 2016-3-5
DOI 10.1016/j.mce.2016.01.006
PII S0303-7207(16)30006-5
PMID 26773729
MeSH Cannabinoid Receptor Agonists / pharmacology Cannabinoid Receptor Antagonists / pharmacology Cannabinoids / pharmacology Cell Line Endocannabinoids / physiology* Estradiol / metabolism Female Follicle Stimulating Hormone / pharmacology Follicle Stimulating Hormone / physiology Gene Expression Granulosa Cells / metabolism* Humans Indoles / pharmacology MicroRNAs / genetics MicroRNAs / metabolism Morpholines / pharmacology Progesterone / pharmacology Progesterone / physiology Pyrazoles / pharmacology Signal Transduction Theca Cells / metabolism
IF 3.563
Resource
Human and Animal Cells KGN (RCB1154)