| RRC ID |
51624
|
| 著者 |
Okubo A, Yasuhira S, Shibazaki M, Takahashi K, Akasaka T, Masuda T, Maesawa C.
|
| タイトル |
NAD(P)H dehydrogenase, quinone 1 (NQO1), protects melanin-producing cells from cytotoxicity of rhododendrol.
|
| ジャーナル |
Pigment Cell Melanoma Res
|
| Abstract |
Rhododendrol (RD) is a potent tyrosinase inhibitor that is metabolized to RD-quinone by tyrosinase, which may underlie the cytotoxicity of RD and leukoderma of the skin that may result. We have examined how forced expression of the NAD(P)H quinone dehydrogenase, quinone 1 (NQO1), a major quinone-reducing enzyme in cytosol, affects the survival of RD-treated cells. We found that treatment of the mouse melanoma cell line B16BL6 or normal human melanocytes with carnosic acid, a transcriptional inducer of the NQO1 gene, notably suppressed the cell killing effect of RD. This effect was mostly abolished by ES936, a highly specific NQO1 inhibitor. Moreover, conditional overexpression of the human NQO1 transgene in B16BL6 led to an expression-dependent increase of cell survival after RD treatment. Our results suggest that NQO1 attenuates the cytotoxicity of RD and/or its metabolites.
|
| 巻・号 |
29(3)
|
| ページ |
309-16
|
| 公開日 |
2016-5-1
|
| DOI |
10.1111/pcmr.12461
|
| PMID |
26847926
|
| MeSH |
Abietanes / pharmacology
Animals
Apoptosis / drug effects
Butanols / pharmacology*
Cell Death / drug effects
Cell Line, Tumor
Cytoprotection / drug effects*
Disease Models, Animal
Humans
Indoleacetic Acids / pharmacology
Melanins / biosynthesis*
Melanocytes / drug effects
Melanocytes / pathology
Mice
NAD(P)H Dehydrogenase (Quinone) / metabolism*
Transcription, Genetic / drug effects
|
| IF |
3.683
|
| 引用数 |
10
|
| リソース情報 |
| ヒト・動物細胞 |
B16/BL6(RCB2638) |
