| RRC ID |
5163
|
| 著者 |
Nagata S, Wang Y, Oshima A, Zhang L, Miyake H, Sasaki H, Ishida A.
|
| タイトル |
Efficient cyclic system to yield ectoine using Brevibacterium sp. JCM 6894 subjected to osmotic downshock.
|
| ジャーナル |
Biotechnol Bioeng
|
| Abstract |
Brevibacterium sp. JCM 6894 cells grown in the presence of 1.5-2.5 M NaCl for 24 h at 30 degrees C were subjected to the osmotic downshock. Downshocked cells after ectoine release were grown for further 24 h in the fresh medium with same salinity as before shock. When this cyclic system was applied to the strain JCM 6894, the amount of ectoine in the cells increased with an increase of incubation time, which indicates that the cells manipulated by the present conditions were enough active to survive and synthesize ectoine after several times of osmotic downshock. In the presence of 2 M NaCl, the highest yield of ectoine released was achieved in this cyclic system, more than 2.4 g/L during 7 days of incubation. (1)H and (13)C-NMR analyses of solutes released from the cells by the osmotic downshock showed the presence of only ectoine with high purity. Release of ectoine from the cells was carried out within 5 min and its rates were increased by the dilution in the downshock treatment. For the convenience of operations, non-sterilized medium containing 2 M NaCl was examined for the cell growth in the present system, in which almost same level of ectoine yield, release rates, and cell viability were observed as those of sterilized medium.
|
| 巻・号 |
99(4)
|
| ページ |
941-8
|
| 公開日 |
2008-3-1
|
| DOI |
10.1002/bit.21619
|
| PMID |
17696218
|
| MeSH |
Amino Acids, Diamino / metabolism*
Brevibacterium / drug effects
Brevibacterium / physiology*
Cell Culture Techniques / methods*
Cell Proliferation / drug effects
Cell Survival / drug effects
Osmotic Pressure / drug effects
Sodium Chloride / pharmacology*
|
| IF |
4.002
|
| 引用数 |
15
|
|
WOS 分野
|
BIOTECHNOLOGY & APPLIED MICROBIOLOGY
|
| リソース情報 |
| 一般微生物 |
JCM6894 |
