論文 - 詳細
| RRC ID | 51651 |
|---|---|
| 著者 | Jdir H, Khemakham B, Najjaa H, Chakroun M, Jridi M, Ben Arfa A, Ben Ali Y, Zouari N. |
| タイトル | Anti-inflammatory and anti-proliferative activities of the wild edible cruciferous: Diplotaxis simplex. |
| ジャーナル | Pharm Biol |
| Abstract |
Context The present study deals with new biological properties of the wild edible Diplotaxis simplex (Viv.) Spreng (Brassicaceae). Objectives The current study evaluates the antioxidant, the anti-inflammatory and the anti-cancer properties of ethyl acetate and ethanol extracts from D. simplex flowers. Materials and methods The anti-proliferative activity of the extracts (10-70 μg/mL) was assessed by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) against human colon cancer cell line Caco-2. The anti-inflammatory potential was evaluated by the inhibitory effect of the extracts (1.5-7.5 mg/mL) on phospholipase A2 activity as well as on carrageenan-induced paw oedema in mice. Extracts (200 mg/kg) or indomethacin (50 mg/kg) as positive control were injected intraperitoneally for albino mice prior to the induction of the oedema by carrageenan. Antioxidant activities were investigated using various complementary methods. Results Flower extracts contained a high level of polyphenolics (17.10-52.70 mg GAE/g) and flavonoids (74.20-100.60 mg QE/g), which correlate with its appreciable antioxidant potential in β-carotene peroxidation (IC50 value: 12.50-27.10 μg/mL), DPPH(•) radical-scavenging (IC50 value: 0.20-0.40 mg/mL), Fe(3+ )reducing (EC50 value: 0.10-0.14 mg/mL) and Fe(2+ )chelating (IC50 value: 0.20-0.60 mg/mL) assays. These extracts were effective in inhibiting cancer cell growth (IC50 value: 62.0-63.25 μg/mL). Besides, the ethyl acetate extract inhibited phospholipase A2 activity (IC50 value: 2.97 mg/mL) and reduced the paw oedema in mice (from 0.38 ± 0.01 to 0.24 ± 0.01 cm), 4 h post-carrageenan challenge. Conclusion These data suggest that D. simplex may be useful as a candidate in the treatment of inflammation and the colon cancer. |
| 巻・号 | 54(10) |
| ページ | 2111-8 |
| 公開日 | 2016-10-1 |
| DOI | 10.3109/13880209.2016.1145704 |
| PMID | 26916801 |
| MeSH | Acetates / chemistry Animals Anti-Infective Agents / isolation & purification Anti-Infective Agents / pharmacology* Antineoplastic Agents, Phytogenic / isolation & purification Antineoplastic Agents, Phytogenic / pharmacology* Antioxidants / isolation & purification Antioxidants / pharmacology Biphenyl Compounds / chemistry Brassicaceae* / chemistry Caco-2 Cells Carrageenan Cell Proliferation / drug effects* Colonic Neoplasms / drug therapy* Colonic Neoplasms / pathology Disease Models, Animal Dose-Response Relationship, Drug Edema / chemically induced Edema / prevention & control* Ethanol / chemistry Flowers Humans Inhibitory Concentration 50 Male Mice Phospholipase A2 Inhibitors / isolation & purification Phospholipase A2 Inhibitors / pharmacology Phospholipases A2 / metabolism Phytotherapy Picrates / chemistry Plant Extracts / isolation & purification Plant Extracts / pharmacology* Plants, Medicinal Solvents / chemistry |
| IF | 2.971 |
| 引用数 | 3 |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 最多言及媒体 | X(Twitter) |
| 各媒体での言及数の合計 | 1 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| ヒト・動物細胞 | CACO-2(RCB0988) |