RRC ID 51651
Author Jdir H, Khemakham B, Najjaa H, Chakroun M, Jridi M, Ben Arfa A, Ben Ali Y, Zouari N.
Title Anti-inflammatory and anti-proliferative activities of the wild edible cruciferous: Diplotaxis simplex.
Journal Pharm Biol
Abstract Context The present study deals with new biological properties of the wild edible Diplotaxis simplex (Viv.) Spreng (Brassicaceae). Objectives The current study evaluates the antioxidant, the anti-inflammatory and the anti-cancer properties of ethyl acetate and ethanol extracts from D. simplex flowers. Materials and methods The anti-proliferative activity of the extracts (10-70 μg/mL) was assessed by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) against human colon cancer cell line Caco-2. The anti-inflammatory potential was evaluated by the inhibitory effect of the extracts (1.5-7.5 mg/mL) on phospholipase A2 activity as well as on carrageenan-induced paw oedema in mice. Extracts (200 mg/kg) or indomethacin (50 mg/kg) as positive control were injected intraperitoneally for albino mice prior to the induction of the oedema by carrageenan. Antioxidant activities were investigated using various complementary methods. Results Flower extracts contained a high level of polyphenolics (17.10-52.70 mg GAE/g) and flavonoids (74.20-100.60 mg QE/g), which correlate with its appreciable antioxidant potential in β-carotene peroxidation (IC50 value: 12.50-27.10 μg/mL), DPPH(•) radical-scavenging (IC50 value: 0.20-0.40 mg/mL), Fe(3+ )reducing (EC50 value: 0.10-0.14 mg/mL) and Fe(2+ )chelating (IC50 value: 0.20-0.60 mg/mL) assays. These extracts were effective in inhibiting cancer cell growth (IC50 value: 62.0-63.25 μg/mL). Besides, the ethyl acetate extract inhibited phospholipase A2 activity (IC50 value: 2.97 mg/mL) and reduced the paw oedema in mice (from 0.38 ± 0.01 to 0.24 ± 0.01 cm), 4 h post-carrageenan challenge. Conclusion These data suggest that D. simplex may be useful as a candidate in the treatment of inflammation and the colon cancer.
Volume 54(10)
Pages 2111-8
Published 2016-10
DOI 10.3109/13880209.2016.1145704
PMID 26916801
MeSH Acetates / chemistry Animals Anti-Infective Agents / isolation & purification Anti-Infective Agents / pharmacology* Antineoplastic Agents, Phytogenic / isolation & purification Antineoplastic Agents, Phytogenic / pharmacology* Antioxidants / isolation & purification Antioxidants / pharmacology Biphenyl Compounds / chemistry Brassicaceae* / chemistry Caco-2 Cells Carrageenan Cell Proliferation / drug effects* Colonic Neoplasms / drug therapy* Colonic Neoplasms / pathology Disease Models, Animal Dose-Response Relationship, Drug Edema / chemically induced Edema / prevention & control* Ethanol / chemistry Flowers Humans Inhibitory Concentration 50 Male Mice Phospholipase A2 Inhibitors / isolation & purification Phospholipase A2 Inhibitors / pharmacology Phospholipases A2 / metabolism Phytotherapy Picrates / chemistry Plant Extracts / isolation & purification Plant Extracts / pharmacology* Plants, Medicinal Solvents / chemistry
IF 2.492
Times Cited 3
Human and Animal Cells CACO-2(RCB0988)