RRC ID 51654
Author Shibahara-Sone H, Gomi A, Iino T, Kano M, Nonaka C, Watanabe O, Miyazaki K, Ohkusa T.
Title Living cells of probiotic Bifidobacterium bifidum YIT 10347 detected on gastric mucosa in humans.
Journal Benef Microbes
Abstract The probiotic strain Bifidobacterium bifidum YIT 10347 has been demonstrated to inhibit Helicobacter pylori activity, prevent injury to the gastric mucosa, and improve general gastric malaise symptoms in H. pylori positive patients. This study aimed to investigate the adhering activity and localisation of B. bifidum YIT 10347 to gastric cells and tissue in vitro, and in human in vivo to clarify the mechanism of its beneficial effects on the stomach. The in vitro study found the adhesion rate of B. bifidum YIT 10347 to human gastric epithelial cells was about 10 times higher than that of lactic acid bacteria and other bifidobacteria. In the human study, 5 H. pylori negative and 12 H. pylori positive subjects ingested milk fermented with B. bifidum YIT 10347. B. bifidum YIT 10347 cells were measured by RT-qPCR for in gastric biopsy samples. Living B. bifidum YIT 10347 cells were detected in the biopsy samples in H. pylori negative subjects (105 cells/g and 104 cells/g at 1 h and 2 h after ingestion, respectively) and H. pylori positive subjects (104 cells/g at 1 h after the ingestion). Moreover, immunostaining analysis of tissue sections found that B. bifidum YIT 10347 cells were located at the interstitial mucin layer of the stomach. These results suggest that cells of probiotic B. bifidum YIT 10347 adhered to the human gastric mucosa in a live state, and that the higher adhering activity of B. bifidum YIT 10347 to the gastric mucosa may be involved in its beneficial effects on the human stomach.
Volume 7(3)
Pages 319-26
Published 2016-6-1
DOI 10.3920/BM2015.0138
PMID 26925600
MeSH Adult Bacterial Adhesion Bacterial Load Bifidobacterium bifidum / isolation & purification* Bifidobacterium bifidum / physiology Biopsy Epithelial Cells / microbiology Female Gastric Mucosa / microbiology* Gastric Mucosa / pathology Healthy Volunteers Helicobacter Infections / pathology Helicobacter Infections / therapy Humans Immunohistochemistry Male Microbial Viability* Middle Aged Probiotics / isolation & purification* Real-Time Polymerase Chain Reaction
IF 3.37
Times Cited 5
Human and Animal Cells GCIY(RCB0555)