Reference - Detail
|Author||Kumagai M, Mishima T, Watanabe A, Harada T, Yoshida I, Fujita K, Watai M, Tawata S, Nishikawa K, Morimoto Y.|
|Title||5,6-Dehydrokawain from Alpinia zerumbet promotes osteoblastic MC3T3-E1 cell differentiation.|
|Journal||Biosci Biotechnol Biochem|
Bone homeostasis is maintained by balancing bone formation and bone resorption, but an imbalance between them is associated with various bone-related diseases such as osteoporosis and rheumatoid arthritis. We found that 5,6-dehydrokawain (DK) and dihydro-5,6-dehydrokawain (DDK), which were isolated as promising compounds from Alpinia zerumbet rhizomes, promote differentiation of osteoblastic MC3T3-E1 cells. DK and DDK increased the alkaline phosphatase activity and matrix mineralization of MC3T3-E1 cells. DK exerts larger effects than DDK. The gene expression of runt-related transcription factor 2 and osterix, which are essential transcription factors in the early period of osteoblast differentiation, was significantly increased by DK treatment. The mRNA level of distal-less homeobox 5 was also enhanced by DK treatment, and DK activated the p38 mitogen-activated protein kinase pathway. Therefore, DK may have clinical potential for preventing osteoporosis, and could be considered as a potential anabolic therapeutic agent.
|MeSH||Alkaline Phosphatase / genetics Alkaline Phosphatase / metabolism Alpinia / chemistry* Animals Cell Differentiation / drug effects* Cell Line Cell Proliferation / drug effects Core Binding Factor Alpha 1 Subunit / agonists Core Binding Factor Alpha 1 Subunit / genetics Core Binding Factor Alpha 1 Subunit / metabolism Gene Expression Regulation Homeodomain Proteins / agonists Homeodomain Proteins / genetics Homeodomain Proteins / metabolism Humans Macrophages / cytology Macrophages / drug effects Macrophages / metabolism Mice Osteoblasts / cytology Osteoblasts / drug effects* Osteoblasts / metabolism Osteogenesis / drug effects* Osteogenesis / genetics Plant Extracts / chemistry Pyrones / isolation & purification Pyrones / pharmacology* RNA, Messenger / agonists RNA, Messenger / genetics RNA, Messenger / metabolism Rhizome / chemistry Sp7 Transcription Factor Transcription Factors / agonists Transcription Factors / genetics Transcription Factors / metabolism p38 Mitogen-Activated Protein Kinases / genetics p38 Mitogen-Activated Protein Kinases / metabolism|
|Human and Animal Cells||MC3T3-E1(RCB1126)|