論文 - 詳細
RRC ID | 51658 |
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著者 | Kumagai M, Mishima T, Watanabe A, Harada T, Yoshida I, Fujita K, Watai M, Tawata S, Nishikawa K, Morimoto Y. |
タイトル | 5,6-Dehydrokawain from Alpinia zerumbet promotes osteoblastic MC3T3-E1 cell differentiation. |
ジャーナル | Biosci Biotechnol Biochem |
Abstract |
Bone homeostasis is maintained by balancing bone formation and bone resorption, but an imbalance between them is associated with various bone-related diseases such as osteoporosis and rheumatoid arthritis. We found that 5,6-dehydrokawain (DK) and dihydro-5,6-dehydrokawain (DDK), which were isolated as promising compounds from Alpinia zerumbet rhizomes, promote differentiation of osteoblastic MC3T3-E1 cells. DK and DDK increased the alkaline phosphatase activity and matrix mineralization of MC3T3-E1 cells. DK exerts larger effects than DDK. The gene expression of runt-related transcription factor 2 and osterix, which are essential transcription factors in the early period of osteoblast differentiation, was significantly increased by DK treatment. The mRNA level of distal-less homeobox 5 was also enhanced by DK treatment, and DK activated the p38 mitogen-activated protein kinase pathway. Therefore, DK may have clinical potential for preventing osteoporosis, and could be considered as a potential anabolic therapeutic agent. |
巻・号 | 80(7) |
ページ | 1425-32 |
公開日 | 2016-7-1 |
DOI | 10.1080/09168451.2016.1153959 |
PMID | 26940726 |
MeSH | Alkaline Phosphatase / genetics Alkaline Phosphatase / metabolism Alpinia / chemistry* Animals Cell Differentiation / drug effects* Cell Line Cell Proliferation / drug effects Core Binding Factor Alpha 1 Subunit / agonists Core Binding Factor Alpha 1 Subunit / genetics Core Binding Factor Alpha 1 Subunit / metabolism Gene Expression Regulation Homeodomain Proteins / agonists Homeodomain Proteins / genetics Homeodomain Proteins / metabolism Humans Macrophages / cytology Macrophages / drug effects Macrophages / metabolism Mice Osteoblasts / cytology Osteoblasts / drug effects* Osteoblasts / metabolism Osteogenesis / drug effects* Osteogenesis / genetics Plant Extracts / chemistry Pyrones / isolation & purification Pyrones / pharmacology* RNA, Messenger / agonists RNA, Messenger / genetics RNA, Messenger / metabolism Rhizome / chemistry Sp7 Transcription Factor Transcription Factors / agonists Transcription Factors / genetics Transcription Factors / metabolism p38 Mitogen-Activated Protein Kinases / genetics p38 Mitogen-Activated Protein Kinases / metabolism |
IF | 1.516 |
引用数 | 9 |
リソース情報 | |
ヒト・動物細胞 | MC3T3-E1(RCB1126) |