RRC ID 51691
Author Darwish AG, Samy MN, Sugimoto S, Otsuka H, Abdel-Salam H, Matsunami K.
Title Effects of Hepatoprotective Compounds from the Leaves of Lumnitzera racemosa on Acetaminophen-Induced Liver Damage in Vitro.
Journal Chem Pharm Bull (Tokyo)
Abstract Phytochemical investigation of the n-BuOH fraction of the mangrove plant Lumnitzera racemosa WILLD. (Combretaceae) led to the isolation of one new flavonoid glycoside; myrcetin 3-O-methyl glucuronate (1), one new phenolic glycoside; lumniracemoside (2) and one new aliphatic alcohol glycoside; n-hexanol 1-O-rutinoside (3), in addition to seven known compounds (4-10). The structures of these compounds were determined by spectroscopic analyses (UV, IR, high resolution-electrospray ionization (HR-ESI)-MS, one- and two-dimensional (1D- and 2D)-NMR). Compound 7 showed the highest hepatoprotective activity against acetaminophen-induced hepatotoxicity using human HepG2 cells at protection % value of 34.2±3.1%, while compounds 1, 2, 3, 6, and 9 showed weak to moderate hepatoprotective activity (11.6-18.9%). Almost all of these compounds showed stronger 1,1-diphenyl-2-picrylhydrazyl (DPPH) radical scavenging activity compared with the standard Trolox. These results suggest the usefulness of this plant extract and the isolated compounds as promising hepatoprotective agents.
Volume 64(4)
Pages 360-5
Published 2016-1-1
DOI 10.1248/cpb.c15-00830
PMID 27039833
MeSH Acetaminophen / toxicity* Chemical and Drug Induced Liver Injury / prevention & control* Combretaceae / chemistry* Hep G2 Cells Humans In Vitro Techniques Liver / drug effects* Magnetic Resonance Spectroscopy Plant Extracts / isolation & purification Plant Extracts / pharmacology* Plant Leaves / chemistry* Spectroscopy, Fourier Transform Infrared
IF 1.416
Times Cited 7
Human and Animal Cells Hep G2(RCB1886)