RRC ID 51714
著者 Nukuda A, Endoh H, Yasuda M, Mizutani T, Kawabata K, Haga H.
タイトル Role of ATF5 in the invasive potential of diverse human cancer cell lines.
ジャーナル Biochem Biophys Res Commun
Abstract Activating transcription factor 5 (ATF5) is a member of the ATF/cAMP response element-binding protein family. Our research group recently revealed that ATF5 expression increases the invasiveness of human lung carcinoma cells. However, the effects of ATF5 on the invasive potential of other cancer cells lines remain unclear. Therefore, in this study, we investigated the role of ATF5 in the invasive activity of diverse human cancer cell lines. Invasiveness was assessed using Matrigel invasion assays. ATF5 knockdown resulted in decreased invasiveness in seven of eight cancer cell lines tested. These results suggest that ATF5 promotes invasiveness in several cancer cell lines. Furthermore, the roles of ATF5 in the invasiveness were evaluated in three-dimensional (3D) culture conditions. In 3D collagen gel, HT-1080 and MDA-MB-231 cells exhibited high invasiveness, with spindle morphology and high invasion speed. In both cell lines, knockdown of ATF5 resulted in rounded morphology and decreased invasion speed. Next, we showed that ATF5 induced integrin-α2 and integrin-β1 expression and that the depletion of integrin-α2 or integrin-β1 resulted in round morphology and decreased invasion speed. Our results suggest that ATF5 promotes invasion by inducing the expression of integrin-α2 and integrin-β1 in several human cancer cell lines.
巻・号 474(3)
ページ 509-514
公開日 2016-6-3
DOI 10.1016/j.bbrc.2016.04.131
PII S0006-291X(16)30645-3
PMID 27125458
MeSH Activating Transcription Factors / metabolism* CD18 Antigens / metabolism* Cell Line, Tumor Gene Expression Regulation, Neoplastic Humans Integrin alpha2 / metabolism* Neoplasm Invasiveness / physiopathology* Neoplasms / metabolism* Neoplasms / pathology*
IF 2.985
引用数 9
リソース情報
ヒト・動物細胞 MKN1(RCB1003)