論文 - 詳細
| RRC ID | 51721 |
|---|---|
| 著者 | Shibata A, Ibaragi S, Mandai H, Tsumura T, Kishimoto K, Okui T, Hassan NM, Shimo T, Omori K, Hu GF, Takashiba S, Suga S, Sasaki A. |
| タイトル | Synthetic Terrein Inhibits Progression of Head and Neck Cancer by Suppressing Angiogenin Production. |
| ジャーナル | Anticancer Res |
| Abstract |
BACKGROUND/AIM:Head and neck cancers are the fifth most common cancer type worldwide, affecting more than half a million patients annually. Development of effective therapeutic drugs is, therefore, required for this type of disease. This study assessed the effects of synthetic terrein on head and neck cancer. MATERIALS AND METHODS:Synthetic terrein was prepared by using the modified Altenhach's procedure. The effect of synthetic terrein on cell proliferation of head and neck cancer cells and HUVECs was assessed. Angiogenin secretion and ribosome biogenesis were examined by ELISA and silver staining of the nucleolar organizer region. A mouse xenograft model was prepared by inoculating mice with suspensions of cells of the human head and neck cancer cell line OSC-19 subcutaneously into the dorsal region of each mouse. Ki-67, CD31 and angiogenin expression in xenografted tumors was examined by immunohistochemistry. RESULTS:Synthetic terrein inhibited the growth of various head and neck cancer cells. In addition, an in vivo experiment revealed that synthetic terrein inhibited a xenograft tumor growth in athymic mice. Immunohistochemical analysis revealed that expression of Ki-67, CD31 and ANG was down-regulated in synthetic terrein-treated tumors, compared to controls. Synthetic terrein suppressed the ANG secretion and ribosome biogenesis in cancer cells, and cell proliferation in vascular endothelial cells. CONCLUSION:The mechanism underlying the anti-tumor effects of synthetic terrein against head and neck cancer consists of the inhibition of both tumor cell proliferation and angiogenesis via the suppression of ANG production. |
| 巻・号 | 36(5) |
| ページ | 2161-8 |
| 公開日 | 2016-5-1 |
| PII | 36/5/2161 |
| PMID | 27127118 |
| MeSH | Antineoplastic Agents / pharmacology* Cell Line, Tumor Cell Proliferation / drug effects Cyclopentanes / pharmacology* Disease Progression Head and Neck Neoplasms / metabolism Head and Neck Neoplasms / pathology* Human Umbilical Vein Endothelial Cells Humans Neovascularization, Pathologic / prevention & control Ribonuclease, Pancreatic / antagonists & inhibitors* Ribonuclease, Pancreatic / biosynthesis |
| IF | 1.994 |
| 引用数 | 8 |
| リソース情報 | |
| ヒト・動物細胞 | HSC-3(RCB1975) HSC-4(RCB1902) |