| RRC ID |
51727
|
| 著者 |
Tamura K, Naraba H, Hara T, Nakamura K, Yoshie M, Kogo H, Tachikawa E.
|
| タイトル |
A positive feedback loop between progesterone and microsomal prostaglandin E synthase-1-mediated PGE2 promotes production of both in mouse granulosa cells.
|
| ジャーナル |
Prostaglandins Other Lipid Mediat
|
| Abstract |
Microsomal prostaglandin E synthase-1 (mPGES-1) is primarily expressed in granulosa cells (GCs) in the preovulatory follicle. Both prostaglandin E2 (PGE2) and progesterone (P4) are implicated in various reproductive functions. Here, we demonstrate that mPges-1 may be a direct downstream target gene of the P4 receptor and P4-stimulated PGE2 secretion can stimulate P4 production in a newly generated mouse GC line (GtsT). Treatment of GtsT cells with a P4 receptor agonist, norgestrel, markedly increased mPGES-1 expression detected by RT-PCR analysis. PGE2 secretion measured by an enzyme-linked immunosorbent assay was enhanced by P4 treatment. Luciferase assays revealed that the proximal promoter region of the mPges-1 gene was responsible for the effects of P4 treatment. Conversely, PGE2 treatment stimulated P4 secretion, which coordinated with mRNA expression of steroidogenic acute regulatory protein. Taken together, P4 may regulate mPGES-1 expression to increase PGE2 secretion and in turn P4 production. An autocrine loop between P4 and PGE2 might function to maintain the increased levels of both in GCs.
|
| 巻・号 |
123
|
| ページ |
56-62
|
| 公開日 |
2016-3-1
|
| DOI |
10.1016/j.prostaglandins.2016.05.002
|
| PII |
S1098-8823(16)30012-0
|
| PMID |
27174800
|
| MeSH |
Animals
Autocrine Communication
Dinoprostone / metabolism*
Dinoprostone / pharmacology
Feedback, Physiological*
Female
Gene Expression Regulation
Genes, Reporter
Granulosa Cells / cytology
Granulosa Cells / drug effects
Granulosa Cells / metabolism*
Luciferases / genetics
Luciferases / metabolism
Mice
Microsomes / metabolism
Norgestrel / pharmacology
Primary Cell Culture
Progesterone / metabolism*
Progesterone / pharmacology
Promoter Regions, Genetic
Prostaglandin-E Synthases / genetics*
Prostaglandin-E Synthases / metabolism
Receptors, Progesterone / agonists
Receptors, Progesterone / genetics*
Receptors, Progesterone / metabolism
Signal Transduction
Tetradecanoylphorbol Acetate / pharmacology
|
| IF |
2.283
|
| 引用数 |
6
|
| リソース情報 |
| ヒト・動物細胞 |
MC3T3-E1(RCB1126) |
