| RRC ID |
51733
|
| Author |
Correia DM, Sencadas V, Ribeiro C, Martins PM, Martins P, Gama FM, Botelho G, Lanceros-Méndez S.
|
| Title |
Processing and size range separation of pristine and magnetic poly(l-lactic acid) based microspheres for biomedical applications.
|
| Journal |
J Colloid Interface Sci
|
| Abstract |
Biodegradable poly(l-lactic acid) (PLLA) and PLLA/CoFe2O4 magnetic microspheres with average sizes ranging between 0.16-3.9μm and 0.8-2.2μm, respectively, were obtained by an oil-in-water emulsion method using poly(vinyl alcohol) (PVA) solution as the emulsifier agent. The separation of the microspheres in different size ranges was then performed by centrifugation and the colloidal stability assessed at different pH values. Neat PLLA spheres are more stable in alkaline environments when compared to magnetic microspheres, both types being stable for pHs higher than 4, resulting in a colloidal suspension. On the other hand, in acidic environments the microspheres tend to form aggregates. The neat PLLA microspheres show a degree of crystallinity of 40% whereas the composite ones are nearly amorphous (17%). Finally, the biocompatibility was assessed by cell viability studies with MC3T3-E1 pre-osteoblast cells.
|
| Volume |
476
|
| Pages |
79-86
|
| Published |
2016-8-15
|
| DOI |
10.1016/j.jcis.2016.05.012
|
| PII |
S0021-9797(16)30297-1
|
| PMID |
27209393
|
| MeSH |
Animals
Biomedical Technology*
Cell Line
Cell Survival / drug effects
Hydrogen-Ion Concentration
Magnetic Phenomena*
Microspheres*
Particle Size
Polyesters / chemistry*
Polyesters / isolation & purification*
Polyesters / pharmacology
Surface Properties
|
| IF |
7.489
|
| Times Cited |
15
|
| Resource |
| Human and Animal Cells |
MC3T3-E1(RCB1126) |
