論文 - 詳細
| RRC ID | 51744 |
|---|---|
| 著者 | Mikami Y, Fukushima A, Komiyama Y, Iwase T, Tsuda H, Higuchi Y, Hayakawa S, Kuyama K, Komiyama K. |
| タイトル | Human uterus myoma and gene expression profiling: A novel in vitro model for studying secretory leukocyte protease inhibitor-mediated tumor invasion. |
| ジャーナル | Cancer Lett |
| Abstract |
Secretory leukocyte protease inhibitor (SLPI) is a serine protease inhibitor that diminishes tissue destruction during inflammation. A recent report revealed high levels of SLPI expression in the oral carcinoma cell. In addition, overexpression of SLPI up-regulates metastasis in lung carcinoma cells. On the other hand, matrix metalloproteinases (MMPs) are proteinases that participate in extracellular matrix degradation. SLPI and MMPs are involved as accelerators of the tumor invasion process; however, their exact roles are not fully understood. Understanding the mechanism of tumor invasion requires models that take the effect of microenvironmental factors into account. In one such in vitro model, different carcinoma cells have been shown to invade myoma tissue in highly distinct patterns. We have used this myoma model, as it provides a more natural stroma-like environment, to investigate the role of SLPI in tumor invasion. Our results indicate that the model provides a relevant matrix for tumor invasion studies, and that SLPI is important for the invasion of oral carcinoma Ca9-22 cells in conjunction with MMPs. Furthermore, using bioinformatics analysis, we have identified candidates as key molecules involved in SLPI-mediated tumor invasion. |
| 巻・号 | 379(1) |
| ページ | 84-93 |
| 公開日 | 2016-8-28 |
| DOI | 10.1016/j.canlet.2016.05.028 |
| PII | S0304-3835(16)30341-X |
| PMID | 27238568 |
| MeSH | Cell Line, Tumor Cell Movement* Coculture Techniques Computational Biology Databases, Genetic Female Gene Expression Profiling* / methods Gene Expression Regulation, Enzymologic Gene Expression Regulation, Neoplastic High-Throughput Nucleotide Sequencing Humans Leiomyoma / enzymology* Leiomyoma / pathology Matrix Metalloproteinase 2 / metabolism Matrix Metalloproteinase 9 / metabolism Mouth Neoplasms / enzymology* Mouth Neoplasms / genetics Mouth Neoplasms / pathology Neoplasm Invasiveness Oligonucleotide Array Sequence Analysis Secretory Leukocyte Peptidase Inhibitor / genetics Secretory Leukocyte Peptidase Inhibitor / metabolism* Signal Transduction Time Factors Tissue Culture Techniques Transfection Tumor Microenvironment Uterine Neoplasms / enzymology* Uterine Neoplasms / pathology |
| IF | 7.36 |
| 引用数 | 1 |
| オルトメトリクス指標 |
オルトメトリクス指標項目
|
| 最多言及媒体 | X(Twitter) |
| 各媒体での言及数の合計 | 1 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| ヒト・動物細胞 | Ca9-22(RCB1976) |