RRC ID 51747
Author Kim H, Park H, Lee JW, Lee KY.
Title Magnetic field-responsive release of transforming growth factor beta 1 from heparin-modified alginate ferrogels.
Journal Carbohydr Polym
Abstract Stimuli-responsive polymeric systems have been widely used for various drug delivery and tissue engineering applications. Magnetic stimulation can be also exploited to regulate the release of pharmaceutical drugs, growth factors, and cells from hydrogels in a controlled manner, on-demand. In the present study, alginate ferrogels containing iron oxide nanoparticles were fabricated via ionic cross-linking, and their various characteristics were investigated. The deformation of the ferrogels was dependent on the polymer concentration, calcium concentration, iron oxide concentration, and strength of magnetic field. To modulate the release of transforming growth factor beta 1 (TGF-β1) under magnetic stimulation, alginate was chemically modified with heparin, as TGF-β1 has a heparin-binding domain. Alginate was first modified with ethylenediamine, and heparin was then conjugated to the ethylenediamine-modified alginate via carbodiimide chemistry. Conjugation of heparin to alginate was confirmed by infrared spectroscopy and proton nuclear magnetic resonance spectroscopy. Sustained release of TGF-β1 from alginate-g-heparin ferrogels was achieved, and application of a magnetic field to the ferrogels regulated TGF-β1 release, resultantly enhancing chondrogenic differentiation of ATDC5 cells, which were used as a model chondrogenic cell line. Alginate-based ferrogels that release drugs in a controlled manner may therefore be useful in many biomedical applications.
Volume 151
Pages 467-473
Published 2016-10-20
DOI 10.1016/j.carbpol.2016.05.090
PII S0144-8617(16)30624-5
PMID 27474590
MeSH Alginates / administration & dosage Alginates / chemistry Animals Cell Differentiation / drug effects Cell Line, Tumor Chondrogenesis / drug effects Drug Delivery Systems* Drug Liberation Glucuronic Acid / administration & dosage Glucuronic Acid / chemistry Heparin / administration & dosage Heparin / chemistry Hexuronic Acids / administration & dosage Hexuronic Acids / chemistry Hydrogels / administration & dosage* Hydrogels / chemistry Magnetic Fields Magnetic Phenomena Magnetite Nanoparticles / administration & dosage Magnetite Nanoparticles / chemistry Mice Transforming Growth Factor beta1 / administration & dosage* Transforming Growth Factor beta1 / chemistry
IF 7.182
Times Cited 18
Human and Animal Cells ATDC5(RCB0565)