Reference - Detail
| RRC ID | 5174 |
|---|---|
| Author | Yamazaki W, Taguchi M, Ishibashi M, Kitazato M, Nukina M, Misawa N, Inoue K. |
| Title | Development and evaluation of a loop-mediated isothermal amplification assay for rapid and simple detection of Campylobacter jejuni and Campylobacter coli. |
| Journal | J Med Microbiol |
| Abstract |
We developed a loop-mediated isothermal amplification (LAMP) assay for the rapid and simple detection of Campylobacter jejuni and Campylobacter coli. The assay provides a specific LAMP product for each of these two species. The assay correctly identified 65 C. jejuni and 45 C. coli strains, but not 75 non-C. jejuni/coli strains. The sensitivity of the LAMP assay for C. jejuni and C. coli in spiked human stool specimens was 5.6 x 10(3) c.f.u. g(-1) (1.4 c.f.u. per test tube) and 4.8 x 10(3) c.f.u. g(-1) (1.2 c.f.u. per test tube), respectively. When 90 stool specimens from patients with diarrhoea were tested by LAMP and direct plating, the LAMP results showed 81.3 % sensitivity and 96.6 % specificity compared to isolation of C. jejuni and C. coli by direct plating. Further, the LAMP assay required less than 2 h for detection of C. jejuni and C. coli in stool specimens. This LAMP assay is a rapid and simple tool for the detection of C. jejuni and C. coli and will be useful in facilitating the early diagnosis of food poisoning incidents caused by these organisms. |
| Volume | 57(Pt 4) |
| Pages | 444-451 |
| Published | 2008-4-1 |
| DOI | 10.1099/jmm.0.47688-0 |
| PMID | 18349363 |
| MeSH | Campylobacter Infections / diagnosis* Campylobacter Infections / microbiology Campylobacter coli / genetics Campylobacter coli / isolation & purification* Campylobacter jejuni / genetics Campylobacter jejuni / isolation & purification* DNA Primers DNA, Bacterial / analysis DNA, Bacterial / isolation & purification Feces / microbiology* Foodborne Diseases / diagnosis* Foodborne Diseases / microbiology Humans Nucleic Acid Amplification Techniques / methods* Sensitivity and Specificity Time Factors |
| IF | 2.156 |
| Times Cited | 57 |
| WOS Category | MICROBIOLOGY |
| Altmetric score |
オルトメトリクス指標項目
|
| The most frequently cited source | Patent(IFI CLAIMS) |
| Total number of mentions | 2 |
| Altmetric score changes over past 6months | 0.0 |
| Resource | |
| General Microbes | JCM2013 JCM2528 JCM2529 JCM2530 |