| RRC ID |
5174
|
| Author |
Yamazaki W, Taguchi M, Ishibashi M, Kitazato M, Nukina M, Misawa N, Inoue K.
|
| Title |
Development and evaluation of a loop-mediated isothermal amplification assay for rapid and simple detection of Campylobacter jejuni and Campylobacter coli.
|
| Journal |
J Med Microbiol
|
| Abstract |
We developed a loop-mediated isothermal amplification (LAMP) assay for the rapid and simple detection of Campylobacter jejuni and Campylobacter coli. The assay provides a specific LAMP product for each of these two species. The assay correctly identified 65 C. jejuni and 45 C. coli strains, but not 75 non-C. jejuni/coli strains. The sensitivity of the LAMP assay for C. jejuni and C. coli in spiked human stool specimens was 5.6 x 10(3) c.f.u. g(-1) (1.4 c.f.u. per test tube) and 4.8 x 10(3) c.f.u. g(-1) (1.2 c.f.u. per test tube), respectively. When 90 stool specimens from patients with diarrhoea were tested by LAMP and direct plating, the LAMP results showed 81.3 % sensitivity and 96.6 % specificity compared to isolation of C. jejuni and C. coli by direct plating. Further, the LAMP assay required less than 2 h for detection of C. jejuni and C. coli in stool specimens. This LAMP assay is a rapid and simple tool for the detection of C. jejuni and C. coli and will be useful in facilitating the early diagnosis of food poisoning incidents caused by these organisms.
|
| Volume |
57(Pt 4)
|
| Pages |
444-451
|
| Published |
2008-4-1
|
| DOI |
10.1099/jmm.0.47688-0
|
| PMID |
18349363
|
| MeSH |
Campylobacter Infections / diagnosis*
Campylobacter Infections / microbiology
Campylobacter coli / genetics
Campylobacter coli / isolation & purification*
Campylobacter jejuni / genetics
Campylobacter jejuni / isolation & purification*
DNA Primers
DNA, Bacterial / analysis
DNA, Bacterial / isolation & purification
Feces / microbiology*
Foodborne Diseases / diagnosis*
Foodborne Diseases / microbiology
Humans
Nucleic Acid Amplification Techniques / methods*
Sensitivity and Specificity
Time Factors
|
| IF |
2.156
|
| Times Cited |
57
|
|
WOS Category
|
MICROBIOLOGY
|
| Resource |
| General Microbes |
JCM2013
JCM2528
JCM2529
JCM2530 |
