論文 - 詳細
| RRC ID | 51755 |
|---|---|
| 著者 | Kurita M, Hanada S, Ichimaru Y, Saito H, Tabata K, Asami S, Miyairi S, Suzuki T. |
| タイトル | Indirubin 3'-Epoxide Induces Caspase-Independent Cell Death in Human Neuroblastoma. |
| ジャーナル | Biol Pharm Bull |
| Abstract |
Indirubin inhibits cyclin-dependent kinases by binding to their ATP-binding site, thereby exerting potent cytotoxicity on some tumor cells. We examined the anti-tumor effect of indirubin 3'-epoxide on human neuroblastoma cell lines (IMR-32, SK-N-SH, and NB-39). The results revealed potent cytotoxicity of indirubin 3'-epoxide against the IMR-32 (IC50: 0.16 µM) and SK-N-SH (IC50: 0.07 µM) cells. Furthermore, it also induced an increase of the sub-G1 population in the IMR-32 cells. Examination by Hoechst 33342 staining revealed apoptosis characterized by cell shrinkage, nuclear condensation and nuclear fragmentation in a concentration-dependent manner. Furthermore, annexin V-propidium iodide (PI) double-staining revealed an increase in the percentage of early apoptotic cells following treatment of the cells with indirubin 3'-epoxide without activation of caspases. In addition, significant decreases in the protein level of survivin and poly(ADP-ribose)polymerase (PARP), and increase in that of apoptosis-inducing factor (AIF) were found in the nuclei of the cells. These results suggest that indirubin 3'-epoxide induced caspase-independent apoptosis through mechanisms involving DNA fragmentation and inhibition of DNA repair. |
| 巻・号 | 39(6) |
| ページ | 993-9 |
| 公開日 | 2016-1-1 |
| DOI | 10.1248/bpb.b15-00999 |
| PMID | 27251501 |
| MeSH | Antineoplastic Agents / pharmacology* Apoptosis / drug effects Apoptosis Regulatory Proteins / metabolism Caspases / metabolism Cell Cycle / drug effects Cell Line, Tumor DNA Fragmentation Humans Indoles / pharmacology* Neuroblastoma / pathology |
| IF | 1.863 |
| 引用数 | 7 |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 最多言及媒体 | Patent(IFI CLAIMS) |
| 各媒体での言及数の合計 | 1 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| ヒト・動物細胞 | SK-N-SH(RCB0426) |