| RRC ID |
51759
|
| 著者 |
Kitagawa M, Ando T, Subarnbhesaj A, Uchida T, Miyauchi M, Takata T.
|
| タイトル |
N-terminal region of human ameloblastin synthetic peptide promotes bone formation.
|
| ジャーナル |
Odontology
|
| Abstract |
The aim of this study was to examine the effect of 16 amino acids of the N-terminal region of human ameloblastin (16N-AMBN) synthetic peptide, on the proliferation and differentiation of MC3T3-E1 cells and bone regeneration. While 16N-AMBN did not affect the proliferation, it induced mRNA expression of type I collagen, alkaline phosphatase (ALP), bone sialoprotein, and osteocalcin. 16N-AMBN also stimulated ALP activity and promoted mineralized nodule formation. On the other hand, these activities were inhibited by anti-16N-AMBN antibody. Treatment of rat calvarial bone defects with 16N-AMBN resulted in almost complete healing compared to that of the control treatments. These findings suggest that 16N-AMBN may be applicable for regeneration therapy of bone defects.
|
| 巻・号 |
105(1)
|
| ページ |
116-121
|
| 公開日 |
2017-1-1
|
| DOI |
10.1007/s10266-016-0243-8
|
| PII |
10.1007/s10266-016-0243-8
|
| PMID |
27262724
|
| MeSH |
Alkaline Phosphatase / metabolism
Animals
Bone Regeneration / drug effects*
Cell Differentiation / drug effects
Cell Line
Cell Proliferation / drug effects
Collagen Type I / metabolism
Dental Enamel Proteins / physiology*
Humans
Integrin-Binding Sialoprotein / metabolism
Osteocalcin / metabolism
Rats
Skull / surgery
Wound Healing / drug effects
|
| IF |
1.84
|
| 引用数 |
1
|
| リソース情報 |
| ヒト・動物細胞 |
MC3T3-E1(RCB1126) |
