| RRC ID |
51797
|
| 著者 |
Kikuchi M, Shimada M, Matsuzaka T, Ishii K, Nakagawa Y, Takayanagi M, Yamada N, Shimano H.
|
| タイトル |
Crucial Role of Elovl6 in Chondrocyte Growth and Differentiation during Growth Plate Development in Mice.
|
| ジャーナル |
PLoS One
|
| Abstract |
ELOVL family member 6, elongation of very long chain fatty acids (Elovl6) is a microsomal enzyme, which regulates the elongation of C12-16 saturated and monounsaturated fatty acids. Elovl6 has been shown to be associated with various pathophysiologies including insulin resistance, atherosclerosis, and non-alcoholic steatohepatitis. To investigate a potential role of Elovl6 during bone development, we here examined a skeletal phenotype of Elovl6 knockout (Elovl6-/-) mice. The Elovl6-/- skeleton was smaller than that of controls, but exhibited no obvious patterning defects. Histological analysis revealed a reduced length of proliferating and an elongated length of hypertrophic chondrocyte layer, and decreased trabecular bone in Elovl6-/- mice compared with controls. These results were presumably due to a modest decrease in chondrocyte proliferation and accelerated differentiation of cells of the chondrocyte lineage. Consistent with the increased length of the hypertrophic chondrocyte layer in Elovl6-/- mice, Collagen10α1 was identified as one of the most affected genes by ablation of Elovl6 in chondrocytes. Furthermore, this elevated expression of Collagen10α1 of Elovl6-null chondrocytes was likely associated with increased levels of Foxa2/a3 and Mef2c mRNA expression. Relative increases in protein levels of nuclear Foxa2 and cytoplasmic histone deacethylase 4/5/7 were also observed in Elovl6 knockdown cells of the chondrocyte lineage. Collectively, our data suggest that Elovl6 plays a critical role for proper development of embryonic growth plate.
|
| 巻・号 |
11(7)
|
| ページ |
e0159375
|
| 公開日 |
2016-1-1
|
| DOI |
10.1371/journal.pone.0159375
|
| PII |
PONE-D-16-12398
|
| PMID |
27467521
|
| PMC |
PMC4965081
|
| MeSH |
Acetyltransferases / genetics
Acetyltransferases / physiology*
Animals
Cell Differentiation / physiology*
Cell Proliferation / physiology*
Chondrocytes / cytology*
Chondrocytes / metabolism
Fatty Acid Elongases
Growth Plate / growth & development*
Mice
Mice, Knockout
Osteoblasts / metabolism
|
| IF |
2.74
|
| 引用数 |
4
|
| リソース情報 |
| ヒト・動物細胞 |
ATDC5(RCB0565) |
