論文 - 詳細
| RRC ID | 51797 |
|---|---|
| 著者 | Kikuchi M, Shimada M, Matsuzaka T, Ishii K, Nakagawa Y, Takayanagi M, Yamada N, Shimano H. |
| タイトル | Crucial Role of Elovl6 in Chondrocyte Growth and Differentiation during Growth Plate Development in Mice. |
| ジャーナル | PLoS One |
| Abstract |
ELOVL family member 6, elongation of very long chain fatty acids (Elovl6) is a microsomal enzyme, which regulates the elongation of C12-16 saturated and monounsaturated fatty acids. Elovl6 has been shown to be associated with various pathophysiologies including insulin resistance, atherosclerosis, and non-alcoholic steatohepatitis. To investigate a potential role of Elovl6 during bone development, we here examined a skeletal phenotype of Elovl6 knockout (Elovl6-/-) mice. The Elovl6-/- skeleton was smaller than that of controls, but exhibited no obvious patterning defects. Histological analysis revealed a reduced length of proliferating and an elongated length of hypertrophic chondrocyte layer, and decreased trabecular bone in Elovl6-/- mice compared with controls. These results were presumably due to a modest decrease in chondrocyte proliferation and accelerated differentiation of cells of the chondrocyte lineage. Consistent with the increased length of the hypertrophic chondrocyte layer in Elovl6-/- mice, Collagen10α1 was identified as one of the most affected genes by ablation of Elovl6 in chondrocytes. Furthermore, this elevated expression of Collagen10α1 of Elovl6-null chondrocytes was likely associated with increased levels of Foxa2/a3 and Mef2c mRNA expression. Relative increases in protein levels of nuclear Foxa2 and cytoplasmic histone deacethylase 4/5/7 were also observed in Elovl6 knockdown cells of the chondrocyte lineage. Collectively, our data suggest that Elovl6 plays a critical role for proper development of embryonic growth plate. |
| 巻・号 | 11(7) |
| ページ | e0159375 |
| 公開日 | 2016-1-1 |
| DOI | 10.1371/journal.pone.0159375 |
| PII | PONE-D-16-12398 |
| PMID | 27467521 |
| PMC | PMC4965081 |
| MeSH | Acetyltransferases / genetics Acetyltransferases / physiology* Animals Cell Differentiation / physiology* Cell Proliferation / physiology* Chondrocytes / cytology* Chondrocytes / metabolism Fatty Acid Elongases Growth Plate / growth & development* Mice Mice, Knockout Osteoblasts / metabolism |
| IF | 2.74 |
| 引用数 | 4 |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 最多言及媒体 | X(Twitter) |
| 各媒体での言及数の合計 | 2 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| ヒト・動物細胞 | ATDC5(RCB0565) |