RRC ID 51811
著者 Miyashita S, Sagane Y, Suzuki T, Matsumoto T, Niwa K, Watanabe T.
タイトル "Non-Toxic" Proteins of the Botulinum Toxin Complex Exert In-vivo Toxicity.
ジャーナル Sci Rep
Abstract The botulinum neurotoxin (BoNT) causes muscle paralysis and is the most potent toxin in nature. BoNT is associated with a complex of auxiliary "Non-Toxic" proteins, which constitute a large-sized toxin complex (L-TC). However, here we report that the "Non-Toxic" complex of serotype D botulinum L-TC, when administered to rats, exerts in-vivo toxicity on small-intestinal villi. Moreover, Serotype C and D of the "Non-Toxic" complex, but not BoNT, induced vacuole-formation in a rat intestinal epithelial cell line (IEC-6), resulting in cell death. Our results suggest that the vacuole was formed in a manner distinct from the mechanism by which Helicobacter pylori vacuolating toxin (VacA) and Vibrio cholerae haemolysin induce vacuolation. We therefore hypothesise that the serotype C and D botulinum toxin complex is a functional hybrid of the neurotoxin and vacuolating toxin (VT) which arose from horizontal gene transfer from an ancestral BoNT-producing bacterium to a hypothetical VT-producing bacterium.
巻・号 6
ページ 31043
公開日 2016-8-10
DOI 10.1038/srep31043
PII srep31043
PMID 27507612
PMC PMC4978960
MeSH Animals Apoptosis Bacterial Proteins / toxicity Botulinum Toxins / toxicity* Cell Line Clostridium botulinum / physiology* Helicobacter pylori / physiology Hemolysin Factors / toxicity Intestinal Mucosa / metabolism* Intestinal Mucosa / ultrastructure Multiprotein Complexes Rats Vacuoles / ultrastructure* Vibrio cholerae / physiology
IF 3.998
引用数 7
リソース情報
ヒト・動物細胞 IEC 6(RCB0993)