論文 - 詳細
| RRC ID | 51811 |
|---|---|
| 著者 | Miyashita S, Sagane Y, Suzuki T, Matsumoto T, Niwa K, Watanabe T. |
| タイトル | "Non-Toxic" Proteins of the Botulinum Toxin Complex Exert In-vivo Toxicity. |
| ジャーナル | Sci Rep |
| Abstract |
The botulinum neurotoxin (BoNT) causes muscle paralysis and is the most potent toxin in nature. BoNT is associated with a complex of auxiliary "Non-Toxic" proteins, which constitute a large-sized toxin complex (L-TC). However, here we report that the "Non-Toxic" complex of serotype D botulinum L-TC, when administered to rats, exerts in-vivo toxicity on small-intestinal villi. Moreover, Serotype C and D of the "Non-Toxic" complex, but not BoNT, induced vacuole-formation in a rat intestinal epithelial cell line (IEC-6), resulting in cell death. Our results suggest that the vacuole was formed in a manner distinct from the mechanism by which Helicobacter pylori vacuolating toxin (VacA) and Vibrio cholerae haemolysin induce vacuolation. We therefore hypothesise that the serotype C and D botulinum toxin complex is a functional hybrid of the neurotoxin and vacuolating toxin (VT) which arose from horizontal gene transfer from an ancestral BoNT-producing bacterium to a hypothetical VT-producing bacterium. |
| 巻・号 | 6 |
| ページ | 31043 |
| 公開日 | 2016-8-10 |
| DOI | 10.1038/srep31043 |
| PII | srep31043 |
| PMID | 27507612 |
| PMC | PMC4978960 |
| MeSH | Animals Apoptosis Bacterial Proteins / toxicity Botulinum Toxins / toxicity* Cell Line Clostridium botulinum / physiology* Helicobacter pylori / physiology Hemolysin Factors / toxicity Intestinal Mucosa / metabolism* Intestinal Mucosa / ultrastructure Multiprotein Complexes Rats Vacuoles / ultrastructure* Vibrio cholerae / physiology |
| IF | 3.998 |
| 引用数 | 7 |
| オルトメトリクス指標 |
オルトメトリクス指標項目
|
| 最多言及媒体 | |
| 各媒体での言及数の合計 | 3 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| ヒト・動物細胞 | IEC 6(RCB0993) |