Reference - Detail
| RRC ID | 51811 |
|---|---|
| Author | Miyashita S, Sagane Y, Suzuki T, Matsumoto T, Niwa K, Watanabe T. |
| Title | "Non-Toxic" Proteins of the Botulinum Toxin Complex Exert In-vivo Toxicity. |
| Journal | Sci Rep |
| Abstract |
The botulinum neurotoxin (BoNT) causes muscle paralysis and is the most potent toxin in nature. BoNT is associated with a complex of auxiliary "Non-Toxic" proteins, which constitute a large-sized toxin complex (L-TC). However, here we report that the "Non-Toxic" complex of serotype D botulinum L-TC, when administered to rats, exerts in-vivo toxicity on small-intestinal villi. Moreover, Serotype C and D of the "Non-Toxic" complex, but not BoNT, induced vacuole-formation in a rat intestinal epithelial cell line (IEC-6), resulting in cell death. Our results suggest that the vacuole was formed in a manner distinct from the mechanism by which Helicobacter pylori vacuolating toxin (VacA) and Vibrio cholerae haemolysin induce vacuolation. We therefore hypothesise that the serotype C and D botulinum toxin complex is a functional hybrid of the neurotoxin and vacuolating toxin (VT) which arose from horizontal gene transfer from an ancestral BoNT-producing bacterium to a hypothetical VT-producing bacterium. |
| Volume | 6 |
| Pages | 31043 |
| Published | 2016-8-10 |
| DOI | 10.1038/srep31043 |
| PII | srep31043 |
| PMID | 27507612 |
| PMC | PMC4978960 |
| MeSH | Animals Apoptosis Bacterial Proteins / toxicity Botulinum Toxins / toxicity* Cell Line Clostridium botulinum / physiology* Helicobacter pylori / physiology Hemolysin Factors / toxicity Intestinal Mucosa / metabolism* Intestinal Mucosa / ultrastructure Multiprotein Complexes Rats Vacuoles / ultrastructure* Vibrio cholerae / physiology |
| IF | 3.998 |
| Times Cited | 7 |
| Altmetric score |
オルトメトリクス指標項目
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| The most frequently cited source | |
| Total number of mentions | 3 |
| Altmetric score changes over past 6months | 0.0 |
| Resource | |
| Human and Animal Cells | IEC 6(RCB0993) |