論文 - 詳細
| RRC ID | 51837 |
|---|---|
| 著者 | Ahmed SR, Takemeura K, Li TC, Kitamoto N, Tanaka T, Suzuki T, Park EY. |
| タイトル | Size-controlled preparation of peroxidase-like graphene-gold nanoparticle hybrids for the visible detection of norovirus-like particles. |
| ジャーナル | Biosens Bioelectron |
| Abstract |
A hybrid structure of graphene-gold nanoparticles (Grp-Au NPs) was designed as a new nanoprobe for colorimetric immunoassays. This hybrid structure was prepared using chloroauric acid, sodium formate and Grp flakes at room temperature. Au NPs attached strongly onto the Grp surface, and their size was controlled by varying the sodium formate concentration. The Raman intensity of the Grp-Au NP hybrids was significantly enhanced at 1567cm-1 and 2730cm-1 compared with those of pristine Grp because of the electronic interaction between Au NPs and Grp. The Grp-Au NPs with a hybrid structure catalyzed the oxidation of the peroxidase substrate 3,3,5,5-tetramethylbenzidine (TMB) with H2O2, developing a blue color in aqueous solution. This catalytic activity was utilized to detect norovirus-like particles (NoV-LPs) in human serum. The enhanced colorimetric response was monitored using Ab-conjugated-Grp-Au NPs and found to depend on the NoV-LP concentration, exhibiting a linear response from 100pg/mL to 10μg/mL. The limit of detection (LOD) of this proposed method was 92.7pg/mL, 112 times lower than that of a conventional enzyme-linked immunosorbent assay (ELISA). The sensitivity of this test was also 41 times greater than that of a commercial diagnostic kit. The selectivity of the Grp-Au NPs was tested with other viruses, and no color changes were observed. Therefore, the proposed system will facilitate the utilization of the intrinsic peroxidase-like activity of Grp-Au NPs in medical diagnostics. We believe that the engineered catalytic Grp-Au NP hybrids could find potential applications in the future development of biocatalysts and bioassays. |
| 巻・号 | 87 |
| ページ | 558-565 |
| 公開日 | 2017-1-15 |
| DOI | 10.1016/j.bios.2016.08.101 |
| PII | S0956-5663(16)30859-4 |
| PMID | 27611475 |
| MeSH | Biosensing Techniques / methods Caliciviridae Infections / blood Caliciviridae Infections / diagnosis* Caliciviridae Infections / virology Colorimetry / methods* Gold / chemistry* Graphite / chemistry* Humans Limit of Detection Nanoparticles / chemistry* Nanoparticles / ultrastructure Norovirus / isolation & purification* Peroxidase / chemistry |
| IF | 10.257 |
| 引用数 | 45 |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 最多言及媒体 | Patent(IFI CLAIMS) |
| 各媒体での言及数の合計 | 1 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| ヒト・動物細胞 | Sf9(RCB0563) |