RRC ID |
51838
|
著者 |
Takeuchi S, Nakano S, Nakamura K, Ozoe A, Chien P, Yoshihara H, Hakuno F, Matsuwaki T, Saeki Y, Takahashi S, Yamanouchi K, Nishihara M.
|
タイトル |
Roles of chondroitin sulfate proteoglycan 4 in fibrogenic/adipogenic differentiation in skeletal muscle tissues.
|
ジャーナル |
Exp Cell Res
|
Abstract |
Intramuscular adipose tissue and fibrous tissue are observed in some skeletal muscle pathologies such as Duchenne muscular dystrophy and sarcopenia, and affect muscle strength and myogenesis. They originate from common fibrogenic/adipogenic cells in the skeletal muscle. Thus, elucidating the regulatory mechanisms underlying fibrogenic/adipogenic cell differentiation is an important step toward the mediation of these disorders. Previously, we established a highly adipogenic progenitor clone, 2G11, from rat skeletal muscle and showed that basic fibroblast growth factor (bFGF) is pro-adipogenic in these cells. Here, we demonstrated that 2G11 cells give rise to fibroblasts upon transforming growth factor (TGF)-β1 stimulation, indicating that they possess mesenchymal progenitor cells (MPC)-like characteristics. The previously reported MPC marker PDGFRα is expressed in other cell populations. Accordingly, we produced monoclonal antibodies that specifically bind to 2G11 cell surface antigens and identified chondroitin sulfate proteoglycan 4 (CSPG4) as a potential MPC marker. Based on an RNA interference analysis, we found that CSPG4 is involved in both the pro-adipogenic effect of bFGF and in TGF-β-induced alpha smooth muscle actin expression and stress fiber formation. By establishing an additional marker for MPC detection and characterizing its role in fibrogenic/adipogenic differentiation, these results will facilitate the development of effective treatments for skeletal muscle pathologies.
|
巻・号 |
347(2)
|
ページ |
367-77
|
公開日 |
2016-10-1
|
DOI |
10.1016/j.yexcr.2016.08.023
|
PII |
S0014-4827(16)30261-0
|
PMID |
27582000
|
MeSH |
Adipogenesis* / drug effects
Animals
Antibodies, Monoclonal / pharmacology
Antigens / metabolism*
Cell Differentiation* / drug effects
Cell Line
Female
Fibroblast Growth Factor 2 / pharmacology
Fibroblasts / cytology*
Fibroblasts / drug effects
Mice, Inbred BALB C
Muscle, Skeletal / cytology*
Proteoglycans / metabolism*
RNA, Small Interfering / metabolism
Rats
Stress Fibers / drug effects
Stress Fibers / metabolism
|
IF |
3.383
|
引用数 |
5
|
リソース情報 |
ヒト・動物細胞 |
P3-X63-Ag8.653(RCB0146) |