RRC ID 51838
著者 Takeuchi S, Nakano S, Nakamura K, Ozoe A, Chien P, Yoshihara H, Hakuno F, Matsuwaki T, Saeki Y, Takahashi S, Yamanouchi K, Nishihara M.
タイトル Roles of chondroitin sulfate proteoglycan 4 in fibrogenic/adipogenic differentiation in skeletal muscle tissues.
ジャーナル Exp Cell Res
Abstract Intramuscular adipose tissue and fibrous tissue are observed in some skeletal muscle pathologies such as Duchenne muscular dystrophy and sarcopenia, and affect muscle strength and myogenesis. They originate from common fibrogenic/adipogenic cells in the skeletal muscle. Thus, elucidating the regulatory mechanisms underlying fibrogenic/adipogenic cell differentiation is an important step toward the mediation of these disorders. Previously, we established a highly adipogenic progenitor clone, 2G11, from rat skeletal muscle and showed that basic fibroblast growth factor (bFGF) is pro-adipogenic in these cells. Here, we demonstrated that 2G11 cells give rise to fibroblasts upon transforming growth factor (TGF)-β1 stimulation, indicating that they possess mesenchymal progenitor cells (MPC)-like characteristics. The previously reported MPC marker PDGFRα is expressed in other cell populations. Accordingly, we produced monoclonal antibodies that specifically bind to 2G11 cell surface antigens and identified chondroitin sulfate proteoglycan 4 (CSPG4) as a potential MPC marker. Based on an RNA interference analysis, we found that CSPG4 is involved in both the pro-adipogenic effect of bFGF and in TGF-β-induced alpha smooth muscle actin expression and stress fiber formation. By establishing an additional marker for MPC detection and characterizing its role in fibrogenic/adipogenic differentiation, these results will facilitate the development of effective treatments for skeletal muscle pathologies.
巻・号 347(2)
ページ 367-77
公開日 2016-10-1
DOI 10.1016/j.yexcr.2016.08.023
PII S0014-4827(16)30261-0
PMID 27582000
MeSH Adipogenesis* / drug effects Animals Antibodies, Monoclonal / pharmacology Antigens / metabolism* Cell Differentiation* / drug effects Cell Line Female Fibroblast Growth Factor 2 / pharmacology Fibroblasts / cytology* Fibroblasts / drug effects Mice, Inbred BALB C Muscle, Skeletal / cytology* Proteoglycans / metabolism* RNA, Small Interfering / metabolism Rats Stress Fibers / drug effects Stress Fibers / metabolism
IF 3.383
引用数 5
リソース情報
ヒト・動物細胞 P3-X63-Ag8.653(RCB0146)