| RRC ID |
51845
|
| 著者 |
Yanaihara N, Noguchi Y, Saito M, Takenaka M, Takakura S, Yamada K, Okamoto A.
|
| タイトル |
MicroRNA Gene Expression Signature Driven by miR-9 Overexpression in Ovarian Clear Cell Carcinoma.
|
| ジャーナル |
PLoS One
|
| Abstract |
Previous studies have identified microRNA (miRNA) involvement in human cancers. This study aimed to elucidate potential clinical and biological associations of ovarian cancer-related miRNA gene expression profiles in high-grade serous carcinoma (HGSC) and ovarian clear cell carcinoma (OCCC). Accordingly, we investigated 27 patients with ovarian cancer (12 HGSC and 15 OCCC cases) using quantitative real-time reverse transcription polymerase chain reaction to determine the cancer-related miRNA expressions. Gene Cluster 3.0 was used for hierarchical clustering analysis, and differentially expressed miRNAs between HGSC and OCCC were identified by the class comparison analysis using BRB-ArrayTools. An unsupervised hierarchical clustering analysis identified two distinct miRNA expression clusters, with histological subtype-related significant differences in the associations between clusters and clinicopathological features. A comparison of miRNA expression in HGSCs and OCCCs identified five miRNAs (miR-132, miR-9, miR-126, miR-34a, and miR-21), with OCCCs demonstrating a statistically higher expression. Further investigation of the biological significance of miR-9 overexpression in OCCC revealed that miR-9 inhibition reduced the cell invasion ability and upregulated E-cadherin expression. Using a luciferase reporter assay, we further demonstrated the direct binding of miR-9 to E-cadherin. Global cancer-related miRNA expression analysis identified statistically unique profiles that could discriminate ovarian cancer histotypes. In OCCC, miR-9 overexpression may affect pathogenesis by targeting E-cadherin, thereby inducing an epithelial-mesenchymal transition. Therefore, miR-9 may be a promising therapeutic target strategy for OCCC.
|
| 巻・号 |
11(9)
|
| ページ |
e0162584
|
| 公開日 |
2016-1-1
|
| DOI |
10.1371/journal.pone.0162584
|
| PII |
PONE-D-16-11139
|
| PMID |
27612152
|
| PMC |
PMC5017778
|
| MeSH |
Adenocarcinoma, Clear Cell / genetics*
Blotting, Western
Cell Line, Tumor
Cell Movement / genetics
Cell Movement / physiology
Female
Humans
In Vitro Techniques
MicroRNAs / genetics*
Middle Aged
Ovarian Neoplasms / genetics*
Reverse Transcriptase Polymerase Chain Reaction
|
| IF |
2.74
|
| 引用数 |
12
|
| リソース情報 |
| ヒト・動物細胞 |
JHOC-5(RCB1520)
JHOC-7(RCB1688)
JHOC-9(RCB2226) |
