RRC ID 51865
著者 Mizukami-Murata S, Sakakibara F, Fujita K, Fukuda M, Kuramata M, Takagi K.
タイトル Detoxification of hydroxylated polychlorobiphenyls by Sphingomonas sp. strain N-9 isolated from forest soil.
ジャーナル Chemosphere
Abstract To examine the biodegradation of hydroxylated polychlorobiphenyls (OH-PCBs), we isolated Sphingomonas sp. strain N-9 from forest soil using mineral salt medium containing 4-hydroxy-3-chlorobiphenyl (4OH-3CB) at the concentration of 10 mg/L. Following incubation with strain N-9, the concentration of 4OH-3CB decreased in inverse proportion to strain N-9 proliferation, and it was converted to 3-chloro-4-hydroxybenzoic acid (4OH-3CBA) after 1 day. We observed that strain N-9 efficiently degraded lowly chlorinated OH-PCBs (1-4 Cl), while highly chlorinated OH-PCBs (5-6 Cl) were less efficiently transformed. Additionally, strain N-9 degraded PCBs and OH-PCBs with similar efficiencies, and the efficiency of OH-PCB degradation was dependent upon the positional relationships between OH-PCB hydroxyl groups and chlorinated rings. OH-PCB biodegradation may result in highly toxic products, therefore, we evaluated the cytotoxicity of two OH-PCBs [4OH-3CB and 4-hydroxy-3,5-dichlorobiphenyl (4OH-3,5CB)] and their metabolites [4OH-3CBA and 3,5-chloro-4-hydroxybenzoic acid (4OH-3,5CBA)] using PC12 rat pheochromocytoma cells. Our results revealed that both OH-PCBs induced cell membrane damage and caused neuron-like elongations in a dose-dependent manner, while similar results were not observed for their metabolites. These results indicated that strain N-9 can convert OH-PCBs into chloro-hydroxybenzoic acids having lower toxicity.
巻・号 165
ページ 173-182
公開日 2016-12-1
DOI 10.1016/j.chemosphere.2016.08.127
PII S0045-6535(16)31162-6
PMID 27649311
MeSH Animals Biodegradation, Environmental Biphenyl Compounds / metabolism* Biphenyl Compounds / toxicity Cell Line, Tumor Chlorobenzoates / metabolism* Chlorobenzoates / toxicity Forests Hydroxybenzoates / metabolism* Hydroxybenzoates / toxicity Hydroxylation Inactivation, Metabolic PC12 Cells Polychlorinated Biphenyls / metabolism* Polychlorinated Biphenyls / toxicity Rats Soil Soil Microbiology Sphingomonas / metabolism*
IF 5.778
引用数 7
リソース情報
ヒト・動物細胞 PC-12(RCB0009)