RRC ID 51886
Author Takano-Yamamoto T, Fukunaga T, Takeshita N.
Title Gene Expression Analysis of CCN Protein in Bone Under Mechanical Stress.
Journal Methods Mol Biol
Abstract To investigate mechanical-dependent bone remodeling, we had previously applied various types of mechanical loading onto the teeth of rats and mice. In vitro cultured bone cells were then used to elucidate the mechanisms underlying the specific phenomenon revealed by in vivo experiments. This review describes the techniques used to upregulate CCN2 expression in bone cells produced by different types of mechanical stress, such as fluid shear stress and substrate strain in vitro, and compression or tension force in vivo.
Volume 1489
Pages 283-308
Published 2017
DOI 10.1007/978-1-4939-6430-7_26
PMID 27734385
MeSH Animals Biomarkers Bite Force Bone and Bones / metabolism* CCN Intercellular Signaling Proteins / genetics* CCN Intercellular Signaling Proteins / metabolism* Fractures, Bone / etiology Fractures, Bone / metabolism Fractures, Bone / pathology Gene Expression* Immunohistochemistry In Situ Hybridization Male Mice Rats Stress, Mechanical*
Times Cited 1
Human and Animal Cells MC3T3-E1(RCB1126)