RRC ID 519
Author Lee JM, Kusakabe T, Kawaguchi Y, Miyagawa Y, Takahashi M, Mon H, Nho SK, Koga K.
Title Molecular cloning and characterization of the translationally controlled tumor protein gene in Bombyx mori.
Journal Comp Biochem Physiol B Biochem Mol Biol
Abstract Translationally controlled tumor protein (Tctp/p23) is known to be synthesized preferentially in cells during the early growth phase of tumors, but is also expressed in normal cells. To elucidate its molecular basis of the expression and physiological significance, a cDNA encoding for the Bombyx mori Tctp (BmTctp) was deduced by editing the partial cDNA sequences registered in a Bombyx EST database. RT-PCR analyses indicated that the BmTCTP mRNA was transcribed in all larval organs examined and was present constantly during the cell cycle of BmN4 cells. A genomic clone of 4255 nucloetide residues produced by inverse PCR contained the 5'-flanking region, two introns and three exons of the BmTCTP gene. Sequence analysis of the 5'-flanking region indicated that a putative promoter region contains several canonical transcription elements such as GATA box, CCAAT motif, MEF2, E4BP4.01 and AP-1, but lacks a TATA box element. Luciferase reporter assay of the deletion constructs of the 5'-flanking region revealed that the -676 to +66 region enhanced the promoter activity the most markedly. In addition to this, there were at least two enhancer-like elements and several repressor elements.
Volume 139(1)
Pages 35-43
Published 2004-9-1
DOI 10.1016/j.cbpc.2004.06.004
PII S1096-4959(04)00157-5
PMID 15364286
MeSH Amino Acid Sequence Animals Base Sequence Biomarkers, Tumor / chemistry Biomarkers, Tumor / genetics* Biomarkers, Tumor / isolation & purification Bombyx / genetics* Cloning, Molecular DNA, Complementary / genetics Expressed Sequence Tags Gamma Rays Genomics Insect Proteins / chemistry Insect Proteins / genetics* Insect Proteins / isolation & purification Molecular Sequence Data Phylogeny Promoter Regions, Genetic / genetics RNA, Messenger / genetics RNA, Messenger / metabolism Sequence Homology Transcription, Genetic / radiation effects
IF 2.219
Times Cited 19
Silkworms r06 EST database