RRC ID 51907
Author Harada K, Ferdous T, Ueyama Y.
Title Gimeracil Exerts Radiosensitizing Effects on Oral Squamous Cell Carcinoma Cells In Vitro and In Vivo.
Journal Anticancer Res
Abstract BACKGROUND/AIM:Gimeracil or 5-chloro-2, 4-dihydroxypyridine (CDHP) has been reported to exert radiosensitization effects in cancer cells by suppressing DNA repair pathways. Here, we investigated the antitumor effect of gimeracil and radiation combination therapy against oral squamous cell carcinoma (OSCC).
MATERIAL AND METHODS:The antitumor activity of gimeracil and/or radiation was investigated in HSC2 and/or SAS cells by growth inhibition assays and clonogenic survival assay. The expression of DNA double-strand break repair proteins were assessed by western blotting and immunohistochemistry, also fluorescent measurements of intracellular reactive oxygen/nitrogen species (ROS/RNS) were carried out in gimeracil and/or radiation-treated HSC2 cells/tumors.
RESULTS:Gimeracil and radiation combination treatment significantly inhibited OSCC cell/tumor growth and colony formation. Down-regulated expressions of DNA double-strand break repair proteins were observed in gimeracil and/or radiation treated cells/tumors. Additionally, the growth inhibitory effect of this combination treatment was associated with reactive oxygen species/reactive nitrogen species (ROS/RNS) generation.
CONCLUSION:Gimeracil might exert radiosensitizing effects on OSCC cells.
Volume 36(11)
Pages 5923-5930
Published 2016-11-1
DOI 10.21873/anticanres.11179
PII 36/11/5923
PMID 27793917
MeSH Animals Carcinoma, Squamous Cell / genetics* Humans In Vitro Techniques Mice Mice, Nude Mouth Neoplasms / pathology* Pyridines / pharmacology* Radiation-Sensitizing Agents / pharmacology*
IF 1.994
Times Cited 3
Resource
Human and Animal Cells HSC-2(RCB1945) SAS(RCB1974)