RRC ID 51936
著者 Pawaputanon Na Mahasarakham C, Izu Y, Nishimori K, Izumi Y, Noda M, Ezura Y.
タイトル Lgr4 Expression in Osteoblastic Cells Is Suppressed by Hydrogen Peroxide Treatment.
ジャーナル J Cell Physiol
Abstract LGR4 is expressed in bone and has been shown to be involved in bone metabolism. Oxidative stress is one of the key issues in pathophysiology of osteoporosis. However, the link between Lgr4 and oxidative stress has not been known. Therefore, effects of hydrogen peroxide on Lgr4 expression in osteoblasts were examined. Hydrogen peroxide treatment suppressed the levels of Lgr4 mRNA expression in an osteoblastic cell line, MC3T3-E1. The suppressive effects were not obvious at 0.1 mM, while 1 mM hydrogen peroxide suppressed Lgr4 expression by more than 50%. Hydrogen peroxide treatment suppressed Lgr4 expression within 12 h and this suppression lasted at least up to 48 h. Hydrogen peroxide suppression of Lgr4 expression was still observed in the presence of a transcription inhibitor but was no longer observed in the presence of a protein synthesis inhibitor. Although Lgr4 expression in osteoblasts is enhanced by BMP2 treatment as reported before, hydrogen peroxide treatment suppressed Lgr4 even in the presence of BMP2. Finally, hydrogen peroxide suppressed Lgr4 expression in primary cultures of osteoblasts similarly to MC3T3-E1 cells. These date indicate that hydrogen peroxide suppresses Lgr4 expression in osteoblastic cells. J. Cell. Physiol. 232: 1761-1766, 2017. © 2016 Wiley Periodicals, Inc.
巻・号 232(7)
ページ 1761-1766
公開日 2017-7-1
DOI 10.1002/jcp.25684
PMID 27861872
MeSH Alkaline Phosphatase / metabolism Animals Cell Line Cytokines / pharmacology Down-Regulation / drug effects Hydrogen Peroxide / toxicity* Mice Osteoblasts / drug effects Osteoblasts / metabolism* Protein Synthesis Inhibitors / pharmacology RNA, Messenger / genetics RNA, Messenger / metabolism RNA, Small Interfering / metabolism Receptors, G-Protein-Coupled / genetics Receptors, G-Protein-Coupled / metabolism* Time Factors Transcription, Genetic / drug effects
IF 4.522
引用数 2
リソース情報
ヒト・動物細胞 MC3T3-E1(RCB1126)