論文 - 詳細
| RRC ID | 51954 |
|---|---|
| 著者 | Onishi T, Tazawa H, Hashimoto Y, Takeuchi M, Otani T, Nakamura S, Sakurai F, Mizuguchi H, Kishimoto H, Umeda Y, Shirakawa Y, Urata Y, Kagawa S, Fujiwara T. |
| タイトル | Tumor-specific delivery of biologics by a novel T-cell line HOZOT. |
| ジャーナル | Sci Rep |
| Abstract |
"Cell-in-cell" denotes an invasive phenotype in which one cell actively internalizes in another. The novel human T-cell line HOZOT, established from human umbilical cord blood, was shown to penetrate a variety of human cancer cells but not normal cells. Oncolytic viruses are emerging as biological therapies for human cancers; however, efficient viral delivery is limited by a lack of tumor-specific homing and presence of pre-existing or therapy-induced neutralizing antibodies. Here, we report a new, intriguing approach using HOZOT cells to transmit biologics such as oncolytic viruses into human cancer cells by cell-in-cell invasion. HOZOT cells were successfully loaded via human CD46 antigen with an attenuated adenovirus containing the fiber protein of adenovirus serotype 35 (OBP-401/F35), in which the telomerase promoter regulates viral replication. OBP-401/F35-loaded HOZOT cells were efficiently internalized into human cancer cells and exhibited tumor-specific killing by release of viruses, even in the presence of anti-viral neutralizing antibodies. Moreover, intraperitoneal administration of HOZOT cells loaded with OBP-401/F35 significantly suppressed peritoneally disseminated tumor growth in mice. This unique cell-in-cell property provides a platform for selective delivery of biologics into human cancer cells, which has important implications for the treatment of human cancers. |
| 巻・号 | 6 |
| ページ | 38060 |
| 公開日 | 2016-11-30 |
| DOI | 10.1038/srep38060 |
| PII | srep38060 |
| PMID | 27901098 |
| PMC | PMC5129011 |
| MeSH | Cell Line, Tumor Genetic Vectors* Humans Membrane Cofactor Protein* Neoplasms / metabolism Neoplasms / pathology Neoplasms / therapy* Oncolytic Virotherapy / methods* Oncolytic Viruses* T-Lymphocytes / virology* |
| IF | 3.998 |
| 引用数 | 3 |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 最多言及媒体 | News |
| 各媒体での言及数の合計 | 25 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| ヒト・動物細胞 | ST2(RCB0224) |