論文 - 詳細
| RRC ID | 51962 |
|---|---|
| 著者 | Domon H, Oda M, Maekawa T, Nagai K, Takeda W, Terao Y. |
| タイトル | Streptococcus pneumoniae disrupts pulmonary immune defence via elastase release following pneumolysin-dependent neutrophil lysis. |
| ジャーナル | Sci Rep |
| Abstract |
Streptococcus pneumoniae is a leading cause of bacterial pneumonia and is the principal cause of morbidity and mortality worldwide. Previous studies suggested that excessive activation of neutrophils results in the release of neutrophil elastase, which contributes to lung injury in severe pneumonia. Although both pneumococcal virulence factors and neutrophil elastase contribute to the development and progression of pneumonia, there are no studies analysing relationships between these factors. Here, we showed that pneumolysin, a pneumococcal pore-forming toxin, induced cell lysis in primary isolated human neutrophils, leading to the release of neutrophil elastase. Pneumolysin exerted minimal cytotoxicity against alveolar epithelial cells and macrophages, whereas neutrophil elastase induced detachment of alveolar epithelial cells and impaired phagocytic activity in macrophages. Additionally, activation of neutrophil elastase did not exert bactericidal activity against S. pneumoniae in vitro. P2X7 receptor, which belongs to a family of purinergic receptors, was involved in pneumolysin-induced cell lysis. These findings suggested that infiltrated neutrophils are the primary target cells of pneumolysin, and that S. pneumoniae exploits neutrophil-elastase leakage to induce the disruption of pulmonary immune defences, thereby causing lung injury. |
| 巻・号 | 6 |
| ページ | 38013 |
| 公開日 | 2016-11-28 |
| DOI | 10.1038/srep38013 |
| PII | srep38013 |
| PMID | 27892542 |
| PMC | PMC5125098 |
| MeSH | Alveolar Epithelial Cells Animals Bacterial Proteins / genetics Bacterial Proteins / metabolism Bacterial Proteins / toxicity Cell Adhesion Cell Death Cell Line Host-Pathogen Interactions / immunology* Humans Leukocyte Elastase / metabolism* Macrophages / microbiology Macrophages / pathology Mice Neutrophils / drug effects Neutrophils / microbiology Neutrophils / pathology* Phagocytosis / physiology Receptors, Purinergic P2X7 / metabolism Streptococcus pneumoniae / pathogenicity* Streptolysins / genetics Streptolysins / metabolism* Streptolysins / toxicity |
| IF | 3.998 |
| 引用数 | 16 |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 最多言及媒体 | Blogs |
| 各媒体での言及数の合計 | 1 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| ヒト・動物細胞 | RAW 264(RCB0535) A549 |