論文 - 詳細
| RRC ID | 51995 |
|---|---|
| 著者 | Suzawa K, Shien K, Peng H, Sakaguchi M, Watanabe M, Hashida S, Maki Y, Yamamoto H, Tomida S, Soh J, Asano H, Tsukuda K, Nasu Y, Kumon H, Miyoshi S, Toyooka S. |
| タイトル | Distant Bystander Effect of REIC/DKK3 Gene Therapy Through Immune System Stimulation in Thoracic Malignancies. |
| ジャーナル | Anticancer Res |
| Abstract |
BACKGROUND:Reduced expression in immortalized cell (REIC)/Dickkoph-3 (DKK3) is a tumor-suppressor gene, and its overexpression by adenovirus vector (Ad-REIC) exhibits a remarkable therapeutic effect on various human cancer types through a mechanism triggered by endoplasmic reticulum stress. MATERIALS AND METHODS:We examined the direct anti-tumor effect of Ad-REIC gene therapy on lung cancer and malignant mesothelioma cell lines in vitro, and the distant bystander effect using immunocompetent mouse allograft models with bilateral flank tumors. RESULTS:Ad-REIC treatment showed antitumor effect in many lung cancer and malignant mesothelioma cell lines in vitro. In an in vivo model, Ad-REIC treatment inhibited the growth not only of directly treated tumors but also of distant untreated tumors. By immunohistochemical analysis, infiltration of T-cells and natural killer (NK) cells and expression of the major histocompatibility complex (MHC) class I molecules were observed in bilateral tumors. CONCLUSION:Ad-REIC treatment not only had a direct antitumor effect but also an indirect bystander effect through stimulation of the immune system. |
| 巻・号 | 37(1) |
| ページ | 301-307 |
| 公開日 | 2017-1-1 |
| DOI | 10.21873/anticanres.11321 |
| PII | 37/1/301 |
| PMID | 28011506 |
| MeSH | Adaptor Proteins, Signal Transducing Adenoviridae / genetics Animals Bystander Effect* Carcinoma, Non-Small-Cell Lung / immunology Carcinoma, Non-Small-Cell Lung / therapy Cell Line, Tumor Chemokines Female Genetic Therapy* Genetic Vectors Humans Intercellular Signaling Peptides and Proteins / genetics* Killer Cells, Natural / immunology Lung Neoplasms / immunology* Lung Neoplasms / therapy* Mesothelioma / immunology Mesothelioma / therapy Mesothelioma, Malignant Mice, Inbred BALB C T-Lymphocytes / immunology |
| IF | 1.994 |
| 引用数 | 3 |
| リソース情報 | |
| ヒト・動物細胞 | KLN205(RCB2623) |