論文 - 詳細
| RRC ID | 52000 |
|---|---|
| 著者 | Hou S, Hang Q, Isaji T, Lu J, Fukuda T, Gu J. |
| タイトル | Importance of membrane-proximal N-glycosylation on integrin β1 in its activation and complex formation. |
| ジャーナル | FASEB J |
| Abstract |
N-Glycosylation of integrin α5β1 plays important roles in cell biologic functions; however, the mechanisms that underlie those roles remain poorly understood. Here, we present evidence that the membrane-proximal N-glycosylation on integrin β1 could positively regulate cell migration by promoting β1 activation. The S4-6 β1 mutant contains only 3 N-glycosylation sites, which are essential for α5 and β1 heterodimer formation, and despite only a small difference in expression levels of α5β1 between wild-type and S4-6 mutant, cell spreading and migration of the S4-6 mutant was significantly decreased compared with that of control. Consistent with these phenotypes, β1-mediated cellular signaling and its activation were clearly suppressed in the S4-6 mutant. Of note, these developments could be rescued by restoration of N-glycosylation sites in the membrane-proximal domain. Further study on the regulatory mechanisms suggested that membrane-proximal N-glycosylation is critical for intermolecular interactions between integrin β1 and other cell membrane proteins, such as syndecan-4 and epidermal growth factor receptor. Moreover, α2,6-sialylation is required for β1 activation. These data suggest a novel regulatory mechanism wherein N-glycosylation near the cell membrane on β1 may serve as a platform that facilitates its complex formation on the cell membrane, thereby affecting integrin-mediated functions.-Hou, S., Hang, Q., Isaji, T., Lu, J., Fukuda, T., Gu, J. Importance of membrane-proximal N-glycosylation on integrin β1 in its activation and complex formation. |
| 巻・号 | 30(12) |
| ページ | 4120-4131 |
| 公開日 | 2016-12-1 |
| DOI | 10.1096/fj.201600665R |
| PII | fj.201600665R |
| PMID | 27565712 |
| MeSH | Animals Cell Adhesion Cell Membrane / metabolism* Cell Movement / physiology* Cricetulus ErbB Receptors / metabolism Glycosylation Humans Integrin alpha5beta1 / metabolism Integrin beta1 / metabolism* Syndecan-4 / metabolism |
| IF | 4.966 |
| 引用数 | 17 |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 最多言及媒体 | X(Twitter) |
| 各媒体での言及数の合計 | 3 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| ヒト・動物細胞 | 293T(RCB2202) HeLa |