| RRC ID |
52041
|
| 著者 |
Nagao M, Tanabe N, Manaka S, Naito M, Sekino J, Takayama T, Kawato T, Torigoe G, Kato S, Tsukune N, Maeno M, Suzuki N, Sato S.
|
| タイトル |
LIPUS suppressed LPS-induced IL-1α through the inhibition of NF-κB nuclear translocation via AT1-PLCβ pathway in MC3T3-E1 cells.
|
| ジャーナル |
J Cell Physiol
|
| Abstract |
Inflammatory cytokines, interleukin (IL)-1, IL-6, and TNF-α, are involved in inflammatory bone diseases such as rheumatoid osteoarthritis and periodontal disease. Particularly, periodontal disease, which destroys alveolar bone, is stimulated by lipopolysaccharide (LPS). Low-intensity pulsed ultrasound (LIPUS) is used for bone healing in orthopedics and dental treatments. However, the mechanism underlying effects of LIPUS on LPS-induced inflammatory cytokine are not well understood. We therefore aimed to investigate the role of LIPUS on LPS-induced IL-1α production. Mouse calvaria osteoblast-like cells MC3T3-E1 were incubated in the presence or absence of LPS (Porphyromonas gingivalis), and then stimulated with LIPUS for 30 min/day. To investigate the role of LIPUS, we determined the expression of IL-1α stimulated with LIPUS and treated with an angiotensin II receptor type 1 (AT1) antagonist, Losartan. We also investigate to clarify the pathway of LIPUS, we transfected siRNA silencing AT1 (siAT1) in MC3T3-E1. LIPUS inhibited mRNA and protein expression of LPS-induced IL-1α. LIPUS also reduced the nuclear translocation of NF-κB by LPS-induced IL-1α. Losartan and siAT1 blocked all the stimulatory effects of LIPUS on IL-1α production and IL-1α-mediated NF-κB translocation induced by LPS. Furthermore, PLCβ inhibitor U73122 recovered NF-κB translocation. These results suggest that LIPUS inhibits LPS-induced IL-1α via AT1-PLCβ in osteoblasts. We exhibit that these findings are in part of the signaling pathway of LIPUS on the anti-inflammatory effects of IL-1α expression.
|
| 巻・号 |
232(12)
|
| ページ |
3337-3346
|
| 公開日 |
2017-12-1
|
| DOI |
10.1002/jcp.25777
|
| PMID |
28063227
|
| MeSH |
Active Transport, Cell Nucleus
Animals
Cell Line
Interleukin-1alpha / metabolism*
Lipopolysaccharides / pharmacology
Mice
NF-kappa B / metabolism*
Phospholipase C beta / metabolism
Receptor, Angiotensin, Type 1 / metabolism
Signal Transduction* / drug effects
Ultrasonic Waves*
|
| IF |
4.522
|
| 引用数 |
15
|
| リソース情報 |
| ヒト・動物細胞 |
MC3T3-E1(RCB1126) |
