| RRC ID |
52044
|
| 著者 |
Hashikura Y, Umeki K, Umekita K, Nomura H, Yamada A, Yamamoto I, Hasegawa H, Yanagihara K, Okayama A.
|
| タイトル |
Infection of defective human T-lymphotropic virus type 1.
|
| ジャーナル |
Hum Cell
|
| Abstract |
In a previous study, we reported that an identical defective provirus had integrated into multiple sites of the genome of a representative human T-lymphotropic virus type 1 (HTLV-1) cell line, MT-2. A possible explanation for this may be the repeated infection of this defective provirus to a cell. Therefore, we attempted to determine whether a defective provirus could transmit during the co-culture of HTLV-1 uninfected human T-cell line, Jurkat, with MT-2 cells treated with mitomycin C. As a result, we established not only a cell line with the integration of one complete provirus, but also a cell line with the integration of one defective provirus. The rearrangement of the T-cell receptor -γ gene of these cell lines showed them to be derived from Jurkat cells. Both HTLV-1 Tax/Rex and HBZ RNA were detected in the cell line, which harbors a complete provirus. On the other hand, HBZ RNA and transcriptional product specific for the defective provirus were detected in the cell line, which harbors a defective HTLV-1 provirus only. These results suggested that a defective HTLV-1 provirus with large depletion of internal sequence could transmit to other cells. Moreover, the defective provirus can be transcriptionally active. This suggested the possibility that the defective HTLV-1 provirus found in the lymphocytes of HTLV-1 carriers and patients with adult T-cell leukemia may transmit to other T-cells in vivo. The results also suggested that defective provirus in HTLV-1 carriers could be functional and may play a role in leukemogenesis.
|
| 巻・号 |
30(2)
|
| ページ |
117-123
|
| 公開日 |
2017-4-1
|
| DOI |
10.1007/s13577-016-0156-4
|
| PII |
10.1007/s13577-016-0156-4
|
| PMID |
28070874
|
| MeSH |
Base Sequence
Cell Line
Gene Rearrangement, T-Lymphocyte
Human T-lymphotropic virus 1 / genetics*
Human T-lymphotropic virus 1 / pathogenicity
Human T-lymphotropic virus 1 / physiology*
Humans
Jurkat Cells / virology*
Polymerase Chain Reaction / methods
Receptors, Antigen, T-Cell, gamma-delta / genetics
Virus Integration*
|
| IF |
2.333
|
| 引用数 |
2
|
| リソース情報 |
| ヒト・動物細胞 |
Jurkat(RCB3052) |
