論文 - 詳細
| RRC ID | 52047 |
|---|---|
| 著者 | Lin HR, Heish CW, Liu CH, Muduli S, Li HF, Higuchi A, Kumar SS, Alarfaj AA, Munusamy MA, Hsu ST, Chen DC, Benelli G, Murugan K, Cheng NC, Wang HC, Wu GJ. |
| タイトル | Purification and differentiation of human adipose-derived stem cells by membrane filtration and membrane migration methods. |
| ジャーナル | Sci Rep |
| Abstract |
Human adipose-derived stem cells (hADSCs) are easily isolated from fat tissue without ethical concerns, but differ in purity, pluripotency, differentiation ability, and stem cell marker expression, depending on the isolation method. We isolated hADSCs from a primary fat tissue solution using: (1) conventional culture, (2) a membrane filtration method, (3) a membrane migration method where the primary cell solution was permeated through membranes, adhered hADSCs were cultured, and hADSCs migrated out from the membranes. Expression of mesenchymal stem cell markers and pluripotency genes, and osteogenic differentiation were compared for hADSCs isolated by different methods using nylon mesh filter membranes with pore sizes ranging from 11 to 80 μm. hADSCs isolated by the membrane migration method had the highest MSC surface marker expression and efficient differentiation into osteoblasts. Osteogenic differentiation ability of hADSCs and MSC surface marker expression were correlated, but osteogenic differentiation ability and pluripotent gene expression were not. |
| 巻・号 | 7 |
| ページ | 40069 |
| 公開日 | 2017-1-10 |
| DOI | 10.1038/srep40069 |
| PII | srep40069 |
| PMID | 28071738 |
| PMC | PMC5223180 |
| MeSH | Adipose Tissue / cytology* Biomarkers / analysis Cell Differentiation* Cell Movement* Cell Separation / methods* Filtration / methods* Humans Stem Cells / physiology* |
| IF | 3.998 |
| 引用数 | 10 |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 最多言及媒体 | X(Twitter) |
| 各媒体での言及数の合計 | 2 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| ヒト・動物細胞 | 454E2(HPS0077) |