Reference - Detail
| RRC ID | 52058 |
|---|---|
| Author | Otaka A, Kitagawa K, Nakaoki T, Hirata M, Fukazawa K, Ishihara K, Mahara A, Yamaoka T. |
| Title | Label-Free Separation of Induced Pluripotent Stem Cells with Anti-SSEA-1 Antibody Immobilized Microfluidic Channel. |
| Journal | Langmuir |
| Abstract |
When induced pluripotent stem cells (iPSCs) are routinely cultured, the obtained cells are a heterogeneous mixture, including feeder cells and partially differentiated cells. Therefore, a purification process is required to use them in a clinical stage. We described a label-free separation of iPSCs using a microfluidic channel. Antibodies against stage-specific embryonic antigen 1 (SSEA-1) was covalently immobilized on the channel coated with a phospholipid polymer. After injection of the heterogeneous cell suspension containing iPSCs, the velocity of cell movement under a liquid flow condition was measured. The mean velocity of the cell movement was 2.1 mm/sec in the unmodified channel, while that in the channel with the immobilized-antibody was 0.4 mm/sec. The eluted cells were fractionated by eluting time. As a result, the SSEA-1 positive iPSCs were mainly contained in later fractions, and the proportion of iPSCs was increased from 43% to 82% as a comparison with the initial cell suspension. These results indicated that iPSCs were selectively separated by the microfluidic channel. This channel is a promising device for label-free separation of iPSCs based on their pluripotent state. |
| Volume | 33(6) |
| Pages | 1576-1582 |
| Published | 2017-2-14 |
| DOI | 10.1021/acs.langmuir.6b04070 |
| PMID | 28092949 |
| MeSH | Animals Antibodies, Monoclonal / chemistry* Cell Separation / methods* Induced Pluripotent Stem Cells / cytology* Lewis X Antigen / immunology* Methacrylates / chemical synthesis Methacrylates / chemistry Mice Microfluidic Analytical Techniques / methods* Polyethylene Glycols / chemical synthesis Polyethylene Glycols / chemistry Polymethacrylic Acids / chemistry |
| IF | 3.557 |
| Times Cited | 6 |
| Altmetric score |
オルトメトリクス指標項目
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| The most frequently cited source | X(Twitter) |
| Total number of mentions | 5 |
| Altmetric score changes over past 6months | 0.0 |
| Resource | |
| Human and Animal Cells | iPS-MEF-Ng-178B-5(APS0002) |