RRC ID 52076
Author Zhao FQ, Glimm DR, Kennelly JJ.
Title Distribution of mammalian facilitative glucose transporter messenger RNA in bovine tissues.
Journal Int J Biochem
Abstract 1. The complementary DNA for five human facilitative glucose transporters (GLUT1, GLUT2, GLUT3, GLUT4 and GLUT5) were used to determine the distribution of facilitative glucose transporter mRNA in bovine tissues by Northern blotting. Under high stringency hybridization conditions, a single 2.8 kb transcript of GLUT1 was seen in all bovine tissues examined except liver. Mammary gland had the highest abundance of GLUT1 mRNA. 2. Four GLUT2 transcripts of 6.3, 3.8, 2.2 and 1.6 kb were observed to be most abundant in liver, with lower abundance in kidney and duodenum. 3. Only a very low level of GLUT3 mRNA was detected in the mammary gland, skeletal muscle and duodenum. 4. Skeletal muscle contained the greatest abundance of GLUT4 mRNA, which was barely detectable in omental fat, kidney and mammary gland. 5. Transcripts of GLUT5 mRNA were detected in relatively high abundance in liver and kidney, and in lower abundance in the duodenum and mammary gland. 6. With the exception of the mammary gland, for which human data have not been reported, the distributions of GLUT1, 2 and 4 mRNA for the bovine tissues examined are similar to that reported for humans. On the other hand, the distributions of GLUT3 and 5 mRNA in the bovine differ from those reported for humans.
Volume 25(12)
Pages 1897-903
Published 1993-12
DOI 10.1016/0020-711x(88)90322-9
PMID 8138027
MeSH Animals Blotting, Northern Cattle / metabolism* Glucose Transporter Type 1 Glucose Transporter Type 2 Glucose Transporter Type 3 Glucose Transporter Type 4 Glucose Transporter Type 5 Humans Monosaccharide Transport Proteins / genetics* Muscle Proteins* Nerve Tissue Proteins* Organ Specificity / physiology RNA, Messenger / metabolism*
Times Cited 75
Human and Animal Cells MRC-5(RCB0211)