RRC ID 52100
Author Koya T, Yanagisawa R, Higuchi Y, Sano K, Shimodaira S.
Title Interferon-α-inducible Dendritic Cells Matured with OK-432 Exhibit TRAIL and Fas Ligand Pathway-mediated Killer Activity.
Journal Sci Rep
Abstract Active human dendritic cells (DCs), which efficiently induce immune responses through their functions as antigen-presenting cells, exhibit direct anti-tumour killing activity in response to some pathogens and cytokines. These antigen-presenting and tumour killing abilities may provide a breakthrough in cancer immunotherapy. However, the mechanisms underlying this killer DC activity have not been fully proven, despite the establishment of interferon-α (IFN-α)-generated killer DCs (IFN-DCs). Here mature IFN-DCs (mIFN-DCs), generated from IFN-DCs primed with OK-432 (streptococcal preparation), exhibited elevated expression of CD86 and human leukocyte antigen-DR (minimum criteria for DC vaccine clinical trials) as well as antigen-presenting abilities comparable with those of mature IL-4-DCs (mIL-4-DCs). Interestingly, the killing activity of mIFN-DCs, which correlated with the expression of CD56 (natural killer cell marker) and was activated via the tumour necrosis factor-related apoptosis-inducing ligand (TRAIL) and Fas ligand pathway, was stronger than that of IFN-DCs and remarkably stronger than that of mIL-4-DCs. Therefore, mIFN-DCs exhibit great potential as an anti-cancer vaccine that would promote both acquired immunity and direct tumour killing.
Volume 7
Pages 42145
Published 2017-2-13
DOI 10.1038/srep42145
PII srep42145
PMID 28191816
PMC PMC5304184
MeSH Antigen Presentation B7-2 Antigen / genetics B7-2 Antigen / immunology CD56 Antigen / genetics CD56 Antigen / immunology Cancer Vaccines / biosynthesis Cell Differentiation / drug effects Cytotoxicity, Immunologic* Dendritic Cells / cytology Dendritic Cells / drug effects* Dendritic Cells / immunology Dinoprostone / pharmacology Fas Ligand Protein / genetics* Fas Ligand Protein / immunology Gene Expression Regulation Granulocyte-Macrophage Colony-Stimulating Factor / pharmacology HLA-DR Antigens / genetics HLA-DR Antigens / immunology Humans Immunophenotyping Interferon-alpha / pharmacology* Interleukin-4 / pharmacology* Monocytes / cytology Monocytes / drug effects Monocytes / immunology Picibanil / pharmacology* Primary Cell Culture Signal Transduction TNF-Related Apoptosis-Inducing Ligand / genetics* TNF-Related Apoptosis-Inducing Ligand / immunology
IF 4.122
Resource
Human and Animal Cells MIA Paca2(RCB2094)