RRC ID 52115
Author Koide N, Kasamatsu A, Endo-Sakamoto Y, Ishida S, Shimizu T, Kimura Y, Miyamoto I, Yoshimura S, Shiiba M, Tanzawa H, Uzawa K.
Title Evidence for Critical Role of Lymphocyte Cytosolic Protein 1 in Oral Cancer.
Journal Sci Rep
Abstract Lymphocyte cytosolic protein 1 (LCP1), a member of actin-binding protein of the plastin family, has been identified in several malignant tumors of non-hematopoietic sites, such as the colon, prostate, and breast. However, little is known about the roles of LCP1 in oral squamous cell carcinomas (OSCCs). This present study sought to clarify the clinical relevance of LCP1 in OSCCs and investigate possible clinical applications for treating OSCCs by regulating LCP1 expression. We found up-regulation of LCP1in OSCCs compared with normal counterparts using real-time quantitative reverse transcription polymerase chain reaction (qRT-PCR), immunoblotting, and immunohistochemistry (P < 0.05). We used shRNA models for LCP1 (shLCP1) and enoxacin (ENX), a fluoroquinolone antibiotic drug, as a regulator of LCP1 expression. In addition to the LCP1 knockdown experiments in which shLCP1 cells showed several depressed functions, including cellular proliferation, invasiveness, and migratory activities, ENX-treated cells also had attenuated functions. Consistent with our hypothesis from our in vitro data, LCP1-positive OSCC samples were correlated closely with the primary tumoral size and regional lymph node metastasis. These results suggested that LCP1 is a useful biomarker for determining progression of OSCCs and that ENX might be a new therapeutic agent for treating OSCCs by controlling LCP1 expression.
Volume 7
Pages 43379
Published 2017-2-23
DOI 10.1038/srep43379
PII srep43379
PMID 28230172
PMC PMC5322526
MeSH Aged Antineoplastic Agents / pharmacology Carcinoma, Squamous Cell / genetics* Carcinoma, Squamous Cell / immunology Carcinoma, Squamous Cell / pathology Cell Line, Tumor Cell Movement / drug effects Cell Proliferation / drug effects Enoxacin / pharmacology Female Gene Expression Regulation, Neoplastic* Humans Lymph Nodes / immunology* Lymph Nodes / pathology Lymphatic Metastasis Male Microfilament Proteins / antagonists & inhibitors Microfilament Proteins / genetics* Microfilament Proteins / immunology Middle Aged Mouth Neoplasms / genetics* Mouth Neoplasms / immunology Mouth Neoplasms / pathology Neoplasm Invasiveness Neoplasm Staging RNA, Small Interfering / genetics RNA, Small Interfering / metabolism T-Lymphocytes / drug effects T-Lymphocytes / immunology* T-Lymphocytes / pathology Tumor Burden
IF 3.998
Times Cited 11
Human and Animal Cells HSC-2(RCB1945) HSC-4(RCB1902) Sa3(RCB0980) Ca9-22(RCB1976) SAS(RCB1974) Ho-1-u-1(RCB2102)