論文 - 詳細
| RRC ID | 52121 |
|---|---|
| 著者 | Hagimori M, Hatabe E, Sano K, Miyazaki H, Sasaki H, Saji H, Mukai T. |
| タイトル | An Activatable Fluorescent γ-Polyglutamic Acid Complex for Sentinel Lymph Node Imaging. |
| ジャーナル | Biol Pharm Bull |
| Abstract |
Sentinel lymph nodes (SLN) are the first lymph nodes (LN) where cancer cells metastasize from the primary tumor. We designed fluorophore-quencher-based activatable nanoparticles for SLN imaging. We selected TAMRA as a fluorophore and BHQ2 or QSY7 as a quencher. Ternary anionic complexes were constructed with generation 4th polyamidoamine dendrimer (G4) modified with TAMRA and p-SCN-Bn-DTPA (DTPA), polyethyleneimine (PEI) modified with BHQ2 or QSY7, and γ-polyglutamic acid (γ-PGA) by the electrostatic self-assembly system. TAMRA-G4-DTPA/PEI-BHQ2/γ-PGA and TAMRA-G4-DTPA/PEI-QSY7/γ-PGA complexes had a particle size of about 40 nm and a ζ-potential of -50 mV, and showed fluorescence resonance energy transfer (FRET) quenching. Fluorescence microscopy studies demonstrated that TAMRA-G4-DTPA/PEI-QSY7/γ-PGA complex produced intracellular fluorescent signals in the lysosome. During in vivo fluorescent imaging, TAMRA-G4-DTPA/PEI-QSY7/γ-PGA complex enabled the detection of mouse popliteal LN. The fluorophore-quencher conjugated γ-PGA complex based on FRET quenching would be useful for fluorescence-based optical imaging of SLN. |
| 巻・号 | 40(3) |
| ページ | 297-302 |
| 公開日 | 2017-1-1 |
| DOI | 10.1248/bpb.b16-00773 |
| PMID | 28250270 |
| MeSH | Animals Dendrimers Fluorescence* Fluorescent Dyes Humans Lymphatic Metastasis / diagnosis Lymphatic Metastasis / diagnostic imaging Lysosomes Male Mice, Inbred BALB C Nanoparticles* Neoplasms / diagnostic imaging* Neoplasms / pathology Optical Imaging / methods* Particle Size Pentetic Acid Polyamines Polyethyleneimine Polyglutamic Acid / analogs & derivatives* Rhodamines Sentinel Lymph Node / diagnostic imaging* |
| IF | 1.863 |
| 引用数 | 4 |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 各媒体での言及数の合計 | 0 |
| リソース情報 | |
| ヒト・動物細胞 | RAW 264(RCB0535) |