| RRC ID |
52121
|
| 著者 |
Hagimori M, Hatabe E, Sano K, Miyazaki H, Sasaki H, Saji H, Mukai T.
|
| タイトル |
An Activatable Fluorescent γ-Polyglutamic Acid Complex for Sentinel Lymph Node Imaging.
|
| ジャーナル |
Biol Pharm Bull
|
| Abstract |
Sentinel lymph nodes (SLN) are the first lymph nodes (LN) where cancer cells metastasize from the primary tumor. We designed fluorophore-quencher-based activatable nanoparticles for SLN imaging. We selected TAMRA as a fluorophore and BHQ2 or QSY7 as a quencher. Ternary anionic complexes were constructed with generation 4th polyamidoamine dendrimer (G4) modified with TAMRA and p-SCN-Bn-DTPA (DTPA), polyethyleneimine (PEI) modified with BHQ2 or QSY7, and γ-polyglutamic acid (γ-PGA) by the electrostatic self-assembly system. TAMRA-G4-DTPA/PEI-BHQ2/γ-PGA and TAMRA-G4-DTPA/PEI-QSY7/γ-PGA complexes had a particle size of about 40 nm and a ζ-potential of -50 mV, and showed fluorescence resonance energy transfer (FRET) quenching. Fluorescence microscopy studies demonstrated that TAMRA-G4-DTPA/PEI-QSY7/γ-PGA complex produced intracellular fluorescent signals in the lysosome. During in vivo fluorescent imaging, TAMRA-G4-DTPA/PEI-QSY7/γ-PGA complex enabled the detection of mouse popliteal LN. The fluorophore-quencher conjugated γ-PGA complex based on FRET quenching would be useful for fluorescence-based optical imaging of SLN.
|
| 巻・号 |
40(3)
|
| ページ |
297-302
|
| 公開日 |
2017-1-1
|
| DOI |
10.1248/bpb.b16-00773
|
| PMID |
28250270
|
| MeSH |
Animals
Dendrimers
Fluorescence*
Fluorescent Dyes
Humans
Lymphatic Metastasis / diagnosis
Lymphatic Metastasis / diagnostic imaging
Lysosomes
Male
Mice, Inbred BALB C
Nanoparticles*
Neoplasms / diagnostic imaging*
Neoplasms / pathology
Optical Imaging / methods*
Particle Size
Pentetic Acid
Polyamines
Polyethyleneimine
Polyglutamic Acid / analogs & derivatives*
Rhodamines
Sentinel Lymph Node / diagnostic imaging*
|
| IF |
1.863
|
| 引用数 |
4
|
| リソース情報 |
| ヒト・動物細胞 |
RAW 264(RCB0535) |
