RRC ID 52137
著者 Juretić M, Jurišić Dukovski B, Krtalić I, Reichl S, Cetina-Čižmek B, Filipović-Grčić J, Lovrić J, Pepić I.
タイトル HCE-T cell-based permeability model: A well-maintained or a highly variable barrier phenotype?
ジャーナル Eur J Pharm Sci
Abstract The most extensively characterized human-derived cell line used in transcorneal permeability studies, in terms of passive transcellular and paracellular transport, transporter expression and metabolic enzymes, is the immortalized human corneal epithelial cell line (HCE-T). The purpose of this study is to describe the changes in the HCE-T barrier phenotype in vitro when valid cultivation conditions, in accordance with the standardized HCE-T cell-based model protocol, were employed. Evaluation of the structural and functional barrier properties revealed two different HCE-T barrier phenotypes, depending on the polycarbonate membrane pore size. Model I (pore size 0.4μm) was characterized by a multilayered HCE-T epithelium at the apical side and a weak barrier function (70-115Ω×cm2), whereas Model II (pore size 3μm) consisted of an apical lipophilic HCE-T monolayer and a basolateral lipophilic monolayer of migrated HCE-T cells that showed improved barrier properties (1700-2600Ω×cm2) compared with Model I. Considering the permeation of ophthalmic compounds and in vitro/ex vivo correlation, Model II was better able to predict transcorneal drug permeation. This study highlights the important aspects of HCE-T barrier phenotype variability that should be continuously monitored in the routine application of HCE-T cell-based models across both academic and pharmaceutical industry research laboratories.
巻・号 104
ページ 23-30
公開日 2017-6-15
DOI 10.1016/j.ejps.2017.03.018
PII S0928-0987(17)30151-3
PMID 28315467
MeSH Animals Cell Line, Transformed Cell Membrane Permeability* Epithelium, Corneal / cytology Epithelium, Corneal / metabolism* Humans In Vitro Techniques Models, Biological* Swine
IF 3.616
引用数 10
リソース情報
ヒト・動物細胞 HCE-T(RCB2280)