RRC ID |
52137
|
著者 |
Juretić M, Jurišić Dukovski B, Krtalić I, Reichl S, Cetina-Čižmek B, Filipović-Grčić J, Lovrić J, Pepić I.
|
タイトル |
HCE-T cell-based permeability model: A well-maintained or a highly variable barrier phenotype?
|
ジャーナル |
Eur J Pharm Sci
|
Abstract |
The most extensively characterized human-derived cell line used in transcorneal permeability studies, in terms of passive transcellular and paracellular transport, transporter expression and metabolic enzymes, is the immortalized human corneal epithelial cell line (HCE-T). The purpose of this study is to describe the changes in the HCE-T barrier phenotype in vitro when valid cultivation conditions, in accordance with the standardized HCE-T cell-based model protocol, were employed. Evaluation of the structural and functional barrier properties revealed two different HCE-T barrier phenotypes, depending on the polycarbonate membrane pore size. Model I (pore size 0.4μm) was characterized by a multilayered HCE-T epithelium at the apical side and a weak barrier function (70-115Ω×cm2), whereas Model II (pore size 3μm) consisted of an apical lipophilic HCE-T monolayer and a basolateral lipophilic monolayer of migrated HCE-T cells that showed improved barrier properties (1700-2600Ω×cm2) compared with Model I. Considering the permeation of ophthalmic compounds and in vitro/ex vivo correlation, Model II was better able to predict transcorneal drug permeation. This study highlights the important aspects of HCE-T barrier phenotype variability that should be continuously monitored in the routine application of HCE-T cell-based models across both academic and pharmaceutical industry research laboratories.
|
巻・号 |
104
|
ページ |
23-30
|
公開日 |
2017-6-15
|
DOI |
10.1016/j.ejps.2017.03.018
|
PII |
S0928-0987(17)30151-3
|
PMID |
28315467
|
MeSH |
Animals
Cell Line, Transformed
Cell Membrane Permeability*
Epithelium, Corneal / cytology
Epithelium, Corneal / metabolism*
Humans
In Vitro Techniques
Models, Biological*
Swine
|
IF |
3.616
|
引用数 |
10
|
リソース情報 |
ヒト・動物細胞 |
HCE-T(RCB2280) |