論文 - 詳細
| RRC ID | 52148 |
|---|---|
| 著者 | Nakahara T, Nishitani Y, Nishiumi S, Yoshida M, Azuma T. |
| タイトル | Astilbin from Engelhardtia chrysolepis enhances intestinal barrier functions in Caco-2 cell monolayers. |
| ジャーナル | Eur J Pharmacol |
| Abstract |
Astilbin, which is one of polyphenolic compounds isolated from the leaves of Engelhardtia chrysolepis HANCE (Chinese name, huang-qui), is available as the effective component in food and cosmetics because of its anti-oxidant and anti-inflammatory effects. The tight junction (TJ) proteins, which protect the body from foreign substances, are related to adhesion between a cell and a cell. Previously, the enhancement of TJ's functions induced by aglycones of flavonoids has been demonstrated, but the effects of the glycosides such as astilbin have not been observed yet. In this study, we investigated the effects of astilbin on the TJ's functions, and human colon carcinoma Caco-2 cell monolayers were used to evaluate the effects of astilbin on transepithelial electrical resistance (TER) value and the mRNA and proteins expressions of TJ-related molecules. Astilbin increased the TER value, mRNA expression levels of claudin-1 and ZO-2, and protein expression levels of occludin and ZO-2 in Caco-2 cells. Astilbin also increased the TER value in Caco-2 cells co-stimulated with TNF-α plus IFN-γ, and moreover upregulated the protein expression of TJ-related molecules in Caco-2 cells co-treated with TNF-α plus IFN-γ. These results suggest that astilbin can enhance the expressions of TJ-related molecules, leading to upregulation of the barrier functions in the intestinal cells. |
| 巻・号 | 804 |
| ページ | 46-51 |
| 公開日 | 2017-6-5 |
| DOI | 10.1016/j.ejphar.2017.03.041 |
| PII | S0014-2999(17)30211-X |
| PMID | 28327343 |
| MeSH | Caco-2 Cells Flavonols / pharmacology* Gene Expression Regulation / drug effects Humans Intestinal Mucosa / drug effects Intestinal Mucosa / metabolism Intestines / cytology* Intestines / drug effects* Juglandaceae / chemistry* Tight Junction Proteins / genetics Tight Junction Proteins / metabolism Tight Junctions / drug effects Tight Junctions / metabolism |
| IF | 3.263 |
| 引用数 | 5 |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 最多言及媒体 | X(Twitter) |
| 各媒体での言及数の合計 | 1 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| ヒト・動物細胞 | CACO-2(RCB0988) |