論文 - 詳細
| RRC ID | 52168 |
|---|---|
| 著者 | Kidani T, Yasuda R, Miyawaki J, Oshima Y, Miura H, Masuno H. |
| タイトル | Bisphenol A Inhibits Cell Proliferation and Reduces the Motile Potential of Murine LM8 Osteosarcoma Cells. |
| ジャーナル | Anticancer Res |
| Abstract |
AIM:The aim of this study was to examine the effect of bisphenol A (BPA) on the proliferation and motility potential of murine LM8 osteosarcoma cells. MATERIALS AND METHODS:LM8 cells were treated for 3 days with or without 80 μM BPA. The effect of BPA on cell proliferation was determined by DNA measurement in the cultures and 5-bromo-2'-deoxyuridine (BrdU) incorporation study. Ethanol-fixed cells were stained with hematoxylin-eosin (H&E) to visualize cell morphology. Cell motility was assayed using inserts with uncoated membranes in invasion chambers. Expression of cell division cycle 42 (CDC42) was determined by immunofluorescence staining and western blotting. RESULTS:BPA reduced the DNA content of cultures and the number of BrdU-positive cells. BPA induced a change in morphology from cuboidal with multiple filopodia on the cell surface to spindle-shaped with a smooth cell surface. BPA-treated cells expressed less CDC42 and were less motile than untreated cells. CONCLUSION:BPA inhibited DNA replication and cell proliferation. BPA inhibited filopodia formation and motile potential by inhibiting CDC42 expression in LM8 cells. |
| 巻・号 | 37(4) |
| ページ | 1711-1722 |
| 公開日 | 2017-4-1 |
| DOI | 10.21873/anticanres.11503 |
| PII | 37/4/1711 |
| PMID | 28373433 |
| MeSH | Animals Apoptosis / drug effects Benzhydryl Compounds / pharmacology* Blotting, Western Bone Neoplasms / drug therapy Bone Neoplasms / metabolism Bone Neoplasms / pathology* Cell Movement / drug effects* Cell Proliferation / drug effects* Estrogens, Non-Steroidal / pharmacology* Fluorescent Antibody Technique Gene Expression Regulation, Neoplastic / drug effects* Mice Osteosarcoma / drug therapy Osteosarcoma / metabolism Osteosarcoma / pathology* Phenols / pharmacology* RNA, Messenger / genetics Real-Time Polymerase Chain Reaction Reverse Transcriptase Polymerase Chain Reaction Tumor Cells, Cultured |
| IF | 1.994 |
| 引用数 | 6 |
| リソース情報 | |
| ヒト・動物細胞 | LM8(RCB1450) |