RRC ID 52202
著者 Fereig RM, Kuroda Y, Terkawi MA, Mahmoud ME, Nishikawa Y.
タイトル Immunization with Toxoplasma gondii peroxiredoxin 1 induces protective immunity against toxoplasmosis in mice.
ジャーナル PLoS One
Abstract To develop a vaccine against Toxoplasma gondii, a vaccine antigen with immune-stimulating activity is required. In the present study, we investigated the immunogenicity and prophylactic potential of T. gondii peroxiredoxin 1 (TgPrx1). The TgPrx1 was detected in the ascitic fluid of mice 6 days postinfection, while specific antibody levels were low in the sera of chronically infected mice. Treatment of murine peritoneal macrophages with recombinant TgPrx1 triggered IL-12p40 and IL-6 production, but not IL-10 production. In response to TgPrx1, activation of NF-kB and IL-6 production were confirmed in mouse macrophage cell line (RAW 264.7). These results suggest the immune-stimulating potentials of TgPrx1. Immunization of mice with recombinant TgPrx1 stimulated specific antibody production (IgG1 and IgG2c). Moreover, spleen cell proliferation and interferon-gamma production significantly increased in the TgPrx1- sensitized cells from mice immunized with the same antigen. Immunization with TgPrx1 also increased mouse survival and decreased cerebral parasite burden against lethal T. gondii infection. Thus, our results suggest that TgPrx1 efficiently induces humoral and cellular immune responses and is useful as a new vaccine antigen against toxoplasmosis.
巻・号 12(4)
ページ e0176324
公開日 2017-4-27
DOI 10.1371/journal.pone.0176324
PII PONE-D-16-42885
PMID 28448521
PMC PMC5407612
MeSH Animals Antibodies, Protozoan / immunology Antibody Specificity Antigens, Protozoan / immunology Female Immunization* Macrophages / immunology Mice Mice, Inbred C57BL Peroxiredoxins / immunology* RAW 264.7 Cells Toxoplasma / immunology* Toxoplasma / physiology* Toxoplasmosis, Animal / prevention & control*
IF 2.74
引用数 3
リソース情報
ヒト・動物細胞 RAW 264(RCB0535)